Involvement of Gα olf-expressing neurons in the vomeronasal system of Bufo japonicus

Kimiko Hagino-Yamagishi, Hideo Nakazawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Most terrestrial vertebrates possess anatomically distinct olfactory organs: the olfactory epithelium (OE) and the vomeronasal organ (VNO). In rodents, olfactory receptors coupled to Gα olf are expressed in the OE, whereas vomeronasal receptors type 1 (V1R) and vomeronasal receptors type 2 (V2R), coupled to Gα i2 and Gα o, respectively, are expressed in the VNO. These receptors and G proteins are thought to play important roles in olfactory perception. However, we previously reported that only V2R and Gα o expression is detected in the Xenopus laevis VNO. As X. laevis spends its entire life in water, we considered that expression of limited types of chemosensory machinery in the VNO might be due to adaptation of the VNO to aquatic life. Thus, we analyzed the expression of G proteins in the VNO and the accessory olfactory bulb (AOB) of the adult Japanese toad, Bufo japonicus, because this species is well adapted to a terrestrial life. By using immunohistochemical analysis in combination with in situ hybridization and DiI labeling, we found that B. japonicus Gα olf and Gα o were expressed in the apical and middle-to-basal layer of the vomeronasal neuroepithelium, and that the axons of these Gα olf- and Gα o-expressing vomeronasal neurons projected to the rostral and caudal accessory olfactory bulb, respectively. These results strongly suggest that both the Gα olf- and Gα o-mediated signal transduction pathways function in the B. japonicus VNO. The expression of Gα olf in the B. japonicus VNO may correlate with the detection of airborne chemical cues and with a terrestrial life.

Original languageEnglish
Pages (from-to)3189-3201
Number of pages13
JournalJournal of Comparative Neurology
Volume519
Issue number16
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Vomeronasal Organ
Bufonidae
Neurons
Olfactory Mucosa
Olfactory Bulb
Xenopus laevis
GTP-Binding Proteins
Olfactory Perception
Odorant Receptors
Anura
In Situ Hybridization
Cues
Axons
Vertebrates
Rodentia
Signal Transduction
Water

Keywords

  • Accessory olfactory bulb
  • Amphibians, lectin
  • G proteins
  • Projection
  • Vomeronasal organ

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Involvement of Gα olf-expressing neurons in the vomeronasal system of Bufo japonicus. / Hagino-Yamagishi, Kimiko; Nakazawa, Hideo.

In: Journal of Comparative Neurology, Vol. 519, No. 16, 01.11.2011, p. 3189-3201.

Research output: Contribution to journalArticle

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