Involvement of hydrogen peroxide production in erbstatin-induced apoptosis in human small cell lung carcinoma cells

Siro Simizu, Masaya Imoto, Noriyuki Masuda, Minoru Takada, Kazuo Umezawa

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Tyrosine kinase inhibitor, erbstatin, induced morphological apoptosis and DNA fragmentation in human small cell lung carcinoma (SCLC) cells. Erbstatin-induced apoptosis was inhibited by antioxidants, whereas erb- statin-inhibited tyrosine phosphorylation was not affected by them. Erbstatin was shown by means of flow cytometry to induce hydrogen peroxide generation. Furthermore, hydrogen peroxide induced morphological apoptosis and DNA fragmentation in the SCLC cells. We also demonstrated that erbstatin-induced hydrogen peroxide production and DNA fragmentation were partially suppressed by inhibition of protein synthesis. Thus, erbstatin-induced apoptosis would be due to hydrogen peroxide generation via newly synthesized protein.

Original languageEnglish
Pages (from-to)4978-4982
Number of pages5
JournalCancer Research
Volume56
Issue number21
Publication statusPublished - 1996 Nov 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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