Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development

Tomoko Taguchi, Hisami Takenouchi, Jun Matsui, Wei Ran Tang, Mitsuko Itagaki, Yusuke Shiozawa, Kyoko Suzuki, Sachi Sakaguchi, Yohko U. Ktagiri, Takao Takahashi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

Original languageEnglish
Pages (from-to)508-518
Number of pages11
JournalExperimental Hematology
Volume34
Issue number4
DOIs
Publication statusPublished - 2006 Apr

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Insulin-Like Growth Factor Binding Proteins
B-Lymphoid Precursor Cells
Insulin-Like Growth Factor I
Bone Marrow Cells
Insulin-Like Growth Factor Binding Protein 6
Insulin-Like Growth Factor Binding Protein 3
Somatomedins
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Recombinant Proteins
Protein Binding
Flow Cytometry
B-Lymphocytes
Cell Count
Cell Proliferation
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Taguchi, T., Takenouchi, H., Matsui, J., Tang, W. R., Itagaki, M., Shiozawa, Y., ... Kiyokawa, N. (2006). Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development. Experimental Hematology, 34(4), 508-518. https://doi.org/10.1016/j.exphem.2006.01.009

Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development. / Taguchi, Tomoko; Takenouchi, Hisami; Matsui, Jun; Tang, Wei Ran; Itagaki, Mitsuko; Shiozawa, Yusuke; Suzuki, Kyoko; Sakaguchi, Sachi; Ktagiri, Yohko U.; Takahashi, Takao; Okita, Hajime; Fujimoto, Junichiro; Kiyokawa, Nobutaka.

In: Experimental Hematology, Vol. 34, No. 4, 04.2006, p. 508-518.

Research output: Contribution to journalArticle

Taguchi, T, Takenouchi, H, Matsui, J, Tang, WR, Itagaki, M, Shiozawa, Y, Suzuki, K, Sakaguchi, S, Ktagiri, YU, Takahashi, T, Okita, H, Fujimoto, J & Kiyokawa, N 2006, 'Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development', Experimental Hematology, vol. 34, no. 4, pp. 508-518. https://doi.org/10.1016/j.exphem.2006.01.009
Taguchi, Tomoko ; Takenouchi, Hisami ; Matsui, Jun ; Tang, Wei Ran ; Itagaki, Mitsuko ; Shiozawa, Yusuke ; Suzuki, Kyoko ; Sakaguchi, Sachi ; Ktagiri, Yohko U. ; Takahashi, Takao ; Okita, Hajime ; Fujimoto, Junichiro ; Kiyokawa, Nobutaka. / Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development. In: Experimental Hematology. 2006 ; Vol. 34, No. 4. pp. 508-518.
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abstract = "Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.",
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AU - Matsui, Jun

AU - Tang, Wei Ran

AU - Itagaki, Mitsuko

AU - Shiozawa, Yusuke

AU - Suzuki, Kyoko

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AU - Ktagiri, Yohko U.

AU - Takahashi, Takao

AU - Okita, Hajime

AU - Fujimoto, Junichiro

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N2 - Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

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