Involvement of NF-κB-mediated maturation of ADAM-17 in the invasion of oral squamous cell carcinoma

Yasuo Takamune, Tetsuro Ikebe, Osamu Nagano, Masanori Shinohara

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The involvement of a disintegrin and metalloprotease domain (ADAM)-17 on the cancer invasion was investigated in oral squamous cell carcinoma (OSCC) cells. TNFα induced the conversion from the proform of ADAM-17 to its mature form time-dependently. TNFα also cleaved CD44 to its small fragments, as observed by a Western blot analysis. The transfection of ADAM-17 siRNA partially suppressed the expression of ADAM-17 as well as the cleavage of CD44. On the other hand, TNFα activated a transcription factor NF-κB in OSCC cells, while NBD peptide, an NF-κB inhibitor, inhibited the ADAM-17 maturation, thus suggesting that NF-κB is involved in ADAM-17 maturation. Moreover, an in vitro invasion assay revealed that both ADAM-17 siRNA and NBD peptides strongly suppressed the TNFα-induced invasion of OSCC cells through the matrix. In conclusion, ADAM-17 plays an important role in cancer invasion probably through CD44 cleavage.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume365
Issue number2
DOIs
Publication statusPublished - 2008 Jan 11
Externally publishedYes

    Fingerprint

Keywords

  • ADAM-17
  • CD44
  • NF-κB
  • Oral cancer
  • Squamous cell carcinoma
  • TNFα

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this