TY - JOUR
T1 - Involvement of NF-κB-mediated maturation of ADAM-17 in the invasion of oral squamous cell carcinoma
AU - Takamune, Yasuo
AU - Ikebe, Tetsuro
AU - Nagano, Osamu
AU - Shinohara, Masanori
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/1/11
Y1 - 2008/1/11
N2 - The involvement of a disintegrin and metalloprotease domain (ADAM)-17 on the cancer invasion was investigated in oral squamous cell carcinoma (OSCC) cells. TNFα induced the conversion from the proform of ADAM-17 to its mature form time-dependently. TNFα also cleaved CD44 to its small fragments, as observed by a Western blot analysis. The transfection of ADAM-17 siRNA partially suppressed the expression of ADAM-17 as well as the cleavage of CD44. On the other hand, TNFα activated a transcription factor NF-κB in OSCC cells, while NBD peptide, an NF-κB inhibitor, inhibited the ADAM-17 maturation, thus suggesting that NF-κB is involved in ADAM-17 maturation. Moreover, an in vitro invasion assay revealed that both ADAM-17 siRNA and NBD peptides strongly suppressed the TNFα-induced invasion of OSCC cells through the matrix. In conclusion, ADAM-17 plays an important role in cancer invasion probably through CD44 cleavage.
AB - The involvement of a disintegrin and metalloprotease domain (ADAM)-17 on the cancer invasion was investigated in oral squamous cell carcinoma (OSCC) cells. TNFα induced the conversion from the proform of ADAM-17 to its mature form time-dependently. TNFα also cleaved CD44 to its small fragments, as observed by a Western blot analysis. The transfection of ADAM-17 siRNA partially suppressed the expression of ADAM-17 as well as the cleavage of CD44. On the other hand, TNFα activated a transcription factor NF-κB in OSCC cells, while NBD peptide, an NF-κB inhibitor, inhibited the ADAM-17 maturation, thus suggesting that NF-κB is involved in ADAM-17 maturation. Moreover, an in vitro invasion assay revealed that both ADAM-17 siRNA and NBD peptides strongly suppressed the TNFα-induced invasion of OSCC cells through the matrix. In conclusion, ADAM-17 plays an important role in cancer invasion probably through CD44 cleavage.
KW - ADAM-17
KW - CD44
KW - NF-κB
KW - Oral cancer
KW - Squamous cell carcinoma
KW - TNFα
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U2 - 10.1016/j.bbrc.2007.11.010
DO - 10.1016/j.bbrc.2007.11.010
M3 - Article
C2 - 17999917
AN - SCOPUS:36248992995
SN - 0006-291X
VL - 365
SP - 393
EP - 398
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
