TY - JOUR
T1 - Involvement of phosphatidylinositol synthesis in the regulation of S phase induction
AU - Imoto, Masaya
AU - Morii, Takanobu
AU - Deguchi, Atsuko
AU - Umezawa, Kazuo
PY - 1994/11
Y1 - 1994/11
N2 - The addition of serum to quiescent normal rat kidney (NRK) cells induced phosphatidylinositol (PI) synthesis after 4 h and DNA synthesis after 16 h. Inostamycin, an inhibitor of CDP-DG:inositol transferase, added within 4 h, inhibited both the serum-induced PI synthesis and S phase entry. By contrast, inostamycin added 8 h after release from quiescence did not inhibit the S phase entry. Inostamycin did not affect the GS/M/G1 transition, and incubation with inostamycin induced cell synchronization at early G1 phase. Thus, PI synthesis is involved in the regulation of S phase induction.
AB - The addition of serum to quiescent normal rat kidney (NRK) cells induced phosphatidylinositol (PI) synthesis after 4 h and DNA synthesis after 16 h. Inostamycin, an inhibitor of CDP-DG:inositol transferase, added within 4 h, inhibited both the serum-induced PI synthesis and S phase entry. By contrast, inostamycin added 8 h after release from quiescence did not inhibit the S phase entry. Inostamycin did not affect the GS/M/G1 transition, and incubation with inostamycin induced cell synchronization at early G1 phase. Thus, PI synthesis is involved in the regulation of S phase induction.
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U2 - 10.1006/excr.1994.1336
DO - 10.1006/excr.1994.1336
M3 - Article
C2 - 7957673
AN - SCOPUS:0028135522
SN - 0014-4827
VL - 215
SP - 228
EP - 233
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -