Involvement of platelet-activating factor in endothelin-induced vascular smooth muscle cell contraction

I. Kurose, S. Miura, M. Suematsu, D. Fukumura, H. Nagata, E. Sekizuka, M. Tsuchiya

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Abstract

This study was designed to elucidate the participation of platelet-activating factor (PAF) in Endothelin-Induced vascular constriction in vivo and in vitro. The microvascular hemodynamic changes in rat mesentery induced by the superfusion of endothelin-1 (ET-1) were visualized through an intravital microscopic system. It was revealed in vivo that ET-1 in a range of 100 fM-10 pM caused a sustained arteriolar constriction in a dosedependent manner. Pretreatment with CV-6209, a selective PAF antagonist, significantly attenuated the constrictive change in arterioles. Changes of intracellular Ca2+mobilization after treatment with ET-1 were investigated in vitro using a cell line (A-10 cell) derived from rat arterial smooth muscle cells. ET-1 caused a prompt rise in the fura-2-associated fluorescence intensity in the individual A-10 cell and it fell to a lower plateau level that was still higher than the baseline value. CV-6209-pre-treated cells did not show the rapid-phase mobilization of Ca2+, but showed the slow late phase of Ca2+activation. The present study demonstrates that PAF may be involved in Endothelin-Induced microvascular constriction by mediating the mobilization of Ca2+in vascular smooth muscle cells.

Original languageEnglish
Pages (from-to)S279-S283
JournalJournal of Cardiovascular Pharmacology
Volume17
Publication statusPublished - 1991 Jan 1

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Keywords

  • Calcium ion (Ca<sup>2+</sup>)
  • Endothelial cell
  • Endothelin-1
  • Microcirculation
  • Platelet-activating factor (PAF)
  • Vascular smooth muscle cell (VSMC)

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Kurose, I., Miura, S., Suematsu, M., Fukumura, D., Nagata, H., Sekizuka, E., & Tsuchiya, M. (1991). Involvement of platelet-activating factor in endothelin-induced vascular smooth muscle cell contraction. Journal of Cardiovascular Pharmacology, 17, S279-S283.