Involvement of signaling through protein kinase C and phosphatidylinositol 3-kinase in the excystation and metacystic development of Entamoeba invadens

Asao Makioka, Masahiro Kumagai, Seiki Kobayashi, Tsutomu Takeuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Using an axenic excystation system in vitro, we examined the effect of protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K), which are signaling molecules responsible for numerous cellular responses, on the excystation and metacystic development of Entamoeba invadens. Excystation, which was assessed by counting the number of metacystic amoebae after the induction of excystation, was inhibited by the PKC inhibitors staurosporine, chelerythrine chloride and calphostin C in a concentration-dependent manner during incubation, compared with the controls. As cyst viability was not affected by these inhibitors, reduced excystation was not due to their direct toxic effects on cysts. Metacystic development, when determined by the number of nuclei in the amoebae, was delayed by these PKC inhibitors, because the percentage of 1-nucleate amoebae was lower than in controls at day 3 of incubation. Wortmannin, a potent inhibitor of PI3K, also inhibited excystation and metacystic development of E. invadens in a concentration-dependent manner, compared with the controls. These results indicate that signaling through PKC and PI3K contributes to the excystation and metacystic development of E. invadens.

Original languageEnglish
Pages (from-to)204-208
Number of pages5
JournalParasitology Research
Volume91
Issue number3
DOIs
Publication statusPublished - 2003 Oct 1

Fingerprint

Phosphatidylinositol 3-Kinase
Entamoeba
excystation
phosphatidylinositol 3-kinase
protein kinase C
Protein Kinase C
Amoeba
Protein C Inhibitor
Protein Kinase Inhibitors
Cysts
Staurosporine
Poisons
Entamoeba invadens
chlorides
viability

ASJC Scopus subject areas

  • Parasitology

Cite this

Involvement of signaling through protein kinase C and phosphatidylinositol 3-kinase in the excystation and metacystic development of Entamoeba invadens. / Makioka, Asao; Kumagai, Masahiro; Kobayashi, Seiki; Takeuchi, Tsutomu.

In: Parasitology Research, Vol. 91, No. 3, 01.10.2003, p. 204-208.

Research output: Contribution to journalArticle

Makioka, Asao ; Kumagai, Masahiro ; Kobayashi, Seiki ; Takeuchi, Tsutomu. / Involvement of signaling through protein kinase C and phosphatidylinositol 3-kinase in the excystation and metacystic development of Entamoeba invadens. In: Parasitology Research. 2003 ; Vol. 91, No. 3. pp. 204-208.
@article{60bcc0b5a3264fdba990a3c7358610b2,
title = "Involvement of signaling through protein kinase C and phosphatidylinositol 3-kinase in the excystation and metacystic development of Entamoeba invadens",
abstract = "Using an axenic excystation system in vitro, we examined the effect of protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K), which are signaling molecules responsible for numerous cellular responses, on the excystation and metacystic development of Entamoeba invadens. Excystation, which was assessed by counting the number of metacystic amoebae after the induction of excystation, was inhibited by the PKC inhibitors staurosporine, chelerythrine chloride and calphostin C in a concentration-dependent manner during incubation, compared with the controls. As cyst viability was not affected by these inhibitors, reduced excystation was not due to their direct toxic effects on cysts. Metacystic development, when determined by the number of nuclei in the amoebae, was delayed by these PKC inhibitors, because the percentage of 1-nucleate amoebae was lower than in controls at day 3 of incubation. Wortmannin, a potent inhibitor of PI3K, also inhibited excystation and metacystic development of E. invadens in a concentration-dependent manner, compared with the controls. These results indicate that signaling through PKC and PI3K contributes to the excystation and metacystic development of E. invadens.",
author = "Asao Makioka and Masahiro Kumagai and Seiki Kobayashi and Tsutomu Takeuchi",
year = "2003",
month = "10",
day = "1",
doi = "10.1007/s00436-003-0955-x",
language = "English",
volume = "91",
pages = "204--208",
journal = "Zeitschrift fur Parasitenkunde",
issn = "0044-3255",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Involvement of signaling through protein kinase C and phosphatidylinositol 3-kinase in the excystation and metacystic development of Entamoeba invadens

AU - Makioka, Asao

AU - Kumagai, Masahiro

AU - Kobayashi, Seiki

AU - Takeuchi, Tsutomu

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Using an axenic excystation system in vitro, we examined the effect of protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K), which are signaling molecules responsible for numerous cellular responses, on the excystation and metacystic development of Entamoeba invadens. Excystation, which was assessed by counting the number of metacystic amoebae after the induction of excystation, was inhibited by the PKC inhibitors staurosporine, chelerythrine chloride and calphostin C in a concentration-dependent manner during incubation, compared with the controls. As cyst viability was not affected by these inhibitors, reduced excystation was not due to their direct toxic effects on cysts. Metacystic development, when determined by the number of nuclei in the amoebae, was delayed by these PKC inhibitors, because the percentage of 1-nucleate amoebae was lower than in controls at day 3 of incubation. Wortmannin, a potent inhibitor of PI3K, also inhibited excystation and metacystic development of E. invadens in a concentration-dependent manner, compared with the controls. These results indicate that signaling through PKC and PI3K contributes to the excystation and metacystic development of E. invadens.

AB - Using an axenic excystation system in vitro, we examined the effect of protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K), which are signaling molecules responsible for numerous cellular responses, on the excystation and metacystic development of Entamoeba invadens. Excystation, which was assessed by counting the number of metacystic amoebae after the induction of excystation, was inhibited by the PKC inhibitors staurosporine, chelerythrine chloride and calphostin C in a concentration-dependent manner during incubation, compared with the controls. As cyst viability was not affected by these inhibitors, reduced excystation was not due to their direct toxic effects on cysts. Metacystic development, when determined by the number of nuclei in the amoebae, was delayed by these PKC inhibitors, because the percentage of 1-nucleate amoebae was lower than in controls at day 3 of incubation. Wortmannin, a potent inhibitor of PI3K, also inhibited excystation and metacystic development of E. invadens in a concentration-dependent manner, compared with the controls. These results indicate that signaling through PKC and PI3K contributes to the excystation and metacystic development of E. invadens.

UR - http://www.scopus.com/inward/record.url?scp=0141921898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141921898&partnerID=8YFLogxK

U2 - 10.1007/s00436-003-0955-x

DO - 10.1007/s00436-003-0955-x

M3 - Article

VL - 91

SP - 204

EP - 208

JO - Zeitschrift fur Parasitenkunde

JF - Zeitschrift fur Parasitenkunde

SN - 0044-3255

IS - 3

ER -