@article{ee661c527e594e7d9074bfd2c8f4e9aa,
title = "Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli",
abstract = "We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the l-cysteine producer. E.coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated l-homocysteine, suggesting that YciW is involved in l-methionine biosynthesis.",
keywords = "Escherichia coli, L-Cysteine, Metabolome analysis, Monobromobimane, YciW gene",
author = "Yusuke Kawano and Iwao Ohtsu and Ai Tamakoshi and Maeka Shiroyama and Ai Tsuruoka and Kyohei Saiki and Kazuhiro Takumi and Gen Nonaka and Tsuyoshi Nakanishi and Takako Hishiki and Makoto Suematsu and Hiroshi Takagi",
note = "Funding Information: Metabolomic analyses were supported partly by Biobank Japan Project from MEXT, Japan. We thank Ajinomoto, Co., Inc. (Tokyo, Japan) for providing the plasmids. This work was supported in part by a grant from Kyowa Hakko Bio Co., Ltd and Ajinomoto Co., Inc (to I.O. and H.T.). This work was also supported by KAKENHI [Grant-in-Aid for Scientific Research (C) (26450091)] from the Japan Society for the Promotion of Science , and by Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry to I.O. Publisher Copyright: {\textcopyright} 2014 The Society for Biotechnology, Japan.",
year = "2015",
month = mar,
day = "1",
doi = "10.1016/j.jbiosc.2014.08.012",
language = "English",
volume = "119",
pages = "310--313",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "Elsevier",
number = "3",
}