Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli

Yusuke Kawano, Iwao Ohtsu, Ai Tamakoshi, Maeka Shiroyama, Ai Tsuruoka, Kyohei Saiki, Kazuhiro Takumi, Gen Nonaka, Tsuyoshi Nakanishi, Takako Hishiki, Makoto Suematsu, Hiroshi Takagi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the l-cysteine producer. E.coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated l-homocysteine, suggesting that YciW is involved in l-methionine biosynthesis.

Original languageEnglish
Pages (from-to)310-313
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume119
Issue number3
DOIs
Publication statusPublished - 2015 Mar 1

Keywords

  • Escherichia coli
  • L-Cysteine
  • Metabolome analysis
  • Monobromobimane
  • YciW gene

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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