Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate

Mayumi Sugimoto, Shinji Sasaki, Toshio Watanabe, Shota Nishimura, Atsushi Ideta, Maya Yamazaki, Keiko Matsuda, Michisuke Yuzaki, Kenji Sakimura, Yoshito Aoyagi, Yoshikazu Sugimoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Ionotropic glutamate receptors mediate most excitatory neurotransmission in the central nervous system by opening ion channels upon the binding of glutamate. Despite the essential roles of glutamate in the control of reproduction and anterior pituitary hormone secretion, there is a limited understanding of how glutamate receptors control ovulation. Here we reveal the function of the ionotropic glutamate receptor AMPA-1 (GRIA1) in ovulation. Based on a genome-wide association study in Bos taurus, we found that ovulation rate is influenced by a variation in the N-terminal leucine/ isoleucine/valine-binding protein (LIVBP) domain of GRIA1, in which serine is replaced by asparagine. GRIA1Asn has a weaker affinity to glutamate than GRIA1Ser, both in Xenopus oocytes and in the membrane fraction of bovine brain. This single amino acid substitution leads to the decreased release of gonadotropin-releasing hormone (GnRH) in immortalized hypothalamic GT1-7 cells. Cows with GRIA1Asn have a slower luteinizing hormone (LH) surge than cows with GRIA1Ser. In addition, cows with GRIA1Asn possess fewer immature ovarian follicles before superovulation and have a lower response to hormone treatment than cows with GRIA1Ser. Our work identified that GRIA1 is a critical mediator of ovulation and that GRIA1 might be a useful target for reproductive therapy.

Original languageEnglish
Article numbere13817
JournalPLoS One
Volume5
Issue number11
DOIs
Publication statusPublished - 2010

Fingerprint

Ovulation
ovulation
Glutamic Acid
glutamates
cows
Anterior Pituitary Hormones
Ionotropic Glutamate Receptors
anterior pituitary hormones
Isoleucine
Asparagine
Glutamate Receptors
Valine
Neurology
Luteinizing Hormone
Superovulation
Ion Channels
Gonadotropin-Releasing Hormone
Leucine
Serine
Ovarian Follicle

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sugimoto, M., Sasaki, S., Watanabe, T., Nishimura, S., Ideta, A., Yamazaki, M., ... Sugimoto, Y. (2010). Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate. PLoS One, 5(11), [e13817]. https://doi.org/10.1371/journal.pone.0013817

Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate. / Sugimoto, Mayumi; Sasaki, Shinji; Watanabe, Toshio; Nishimura, Shota; Ideta, Atsushi; Yamazaki, Maya; Matsuda, Keiko; Yuzaki, Michisuke; Sakimura, Kenji; Aoyagi, Yoshito; Sugimoto, Yoshikazu.

In: PLoS One, Vol. 5, No. 11, e13817, 2010.

Research output: Contribution to journalArticle

Sugimoto, M, Sasaki, S, Watanabe, T, Nishimura, S, Ideta, A, Yamazaki, M, Matsuda, K, Yuzaki, M, Sakimura, K, Aoyagi, Y & Sugimoto, Y 2010, 'Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate', PLoS One, vol. 5, no. 11, e13817. https://doi.org/10.1371/journal.pone.0013817
Sugimoto M, Sasaki S, Watanabe T, Nishimura S, Ideta A, Yamazaki M et al. Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate. PLoS One. 2010;5(11). e13817. https://doi.org/10.1371/journal.pone.0013817
Sugimoto, Mayumi ; Sasaki, Shinji ; Watanabe, Toshio ; Nishimura, Shota ; Ideta, Atsushi ; Yamazaki, Maya ; Matsuda, Keiko ; Yuzaki, Michisuke ; Sakimura, Kenji ; Aoyagi, Yoshito ; Sugimoto, Yoshikazu. / Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate. In: PLoS One. 2010 ; Vol. 5, No. 11.
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