IRF-1 mediates upregulation of LMP7 by IFN-γ and concerted expression of immunosubunits of the proteasome

Shin Namiki, Tetsuya Nakamura, Shigeru Oshima, Motomi Yamazaki, Yuko Sekine, Kiichiro Tsuchiya, Ryuichi Okamoto, Takanori Kanai, Mamoru Watanabe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

An immunoproteasome subunit low molecular weight protein 7 (LMP7) plays critical roles in major histocompatibility complex class I antigen processing; however, the mechanism for its expression has remained unclear. We demonstrate that interferon (IFN) regulatory factor-1 (IRF-1) has a pivotal role in IFN-γ-dependent LMP7 expression, as was shown for the other two immunosubunits. A tetracycline-inducible system for IRF-1 revealed its function in the LMP7 expression, and a genomic region functionally interacting with IRF-1 was also determined. Furthermore, the role of IRF-1 in IFN-γ-inducible LMP7 transcription was confirmed by employing small interfering RNA experiments and IRF-1-/- mice. These results suggest that IRF-1 acts as a master regulator for the concerted expression of immunoproteasome components.

Original languageEnglish
Pages (from-to)2781-2787
Number of pages7
JournalFEBS Letters
Volume579
Issue number13
DOIs
Publication statusPublished - 2005 May 23
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
Interferons
Up-Regulation
Molecular Weight
Molecular weight
Interferon Regulatory Factor-1
Proteins
Histocompatibility Antigens Class I
Antigen Presentation
Transcription
Tetracycline
Major Histocompatibility Complex
Small Interfering RNA
Processing
Experiments

Keywords

  • Immunoproteasome
  • Interferon regulatory factor-1
  • Low molecular weight protein 2
  • Low molecular weight protein 7
  • Major histocompatibility complex class I antigen processing
  • Multicatalytic endopeptidase complex-like 1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Namiki, S., Nakamura, T., Oshima, S., Yamazaki, M., Sekine, Y., Tsuchiya, K., ... Watanabe, M. (2005). IRF-1 mediates upregulation of LMP7 by IFN-γ and concerted expression of immunosubunits of the proteasome. FEBS Letters, 579(13), 2781-2787. https://doi.org/10.1016/j.febslet.2005.04.012

IRF-1 mediates upregulation of LMP7 by IFN-γ and concerted expression of immunosubunits of the proteasome. / Namiki, Shin; Nakamura, Tetsuya; Oshima, Shigeru; Yamazaki, Motomi; Sekine, Yuko; Tsuchiya, Kiichiro; Okamoto, Ryuichi; Kanai, Takanori; Watanabe, Mamoru.

In: FEBS Letters, Vol. 579, No. 13, 23.05.2005, p. 2781-2787.

Research output: Contribution to journalArticle

Namiki, S, Nakamura, T, Oshima, S, Yamazaki, M, Sekine, Y, Tsuchiya, K, Okamoto, R, Kanai, T & Watanabe, M 2005, 'IRF-1 mediates upregulation of LMP7 by IFN-γ and concerted expression of immunosubunits of the proteasome', FEBS Letters, vol. 579, no. 13, pp. 2781-2787. https://doi.org/10.1016/j.febslet.2005.04.012
Namiki, Shin ; Nakamura, Tetsuya ; Oshima, Shigeru ; Yamazaki, Motomi ; Sekine, Yuko ; Tsuchiya, Kiichiro ; Okamoto, Ryuichi ; Kanai, Takanori ; Watanabe, Mamoru. / IRF-1 mediates upregulation of LMP7 by IFN-γ and concerted expression of immunosubunits of the proteasome. In: FEBS Letters. 2005 ; Vol. 579, No. 13. pp. 2781-2787.
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