IRF4 Transcription Factor-Dependent CD11b+ Dendritic Cells in Human and Mouse Control Mucosal IL-17 Cytokine Responses

Andreas Schlitzer, Naomi McGovern, Pearline Teo, Teresa Zelante, Koji Atarashi, Donovan Low, AdrianW S. Ho, Peter See, Amanda Shin, PavandipSingh Wasan, Guillaume Hoeffel, Benoit Malleret, Alexander Heiseke, Samantha Chew, Laura Jardine, HarrietA Purvis, CatharienM U. Hilkens, John Tam, Michael Poidinger, E. Richard Stanley & 9 others AnneB Krug, Laurent Renia, Baalasubramanian Sivasankar, LaiGuan Ng, Matthew Collin, Paola Ricciardi-Castagnoli, Kenya Honda, Muzlifah Haniffa, Florent Ginhoux

Research output: Contribution to journalArticle

411 Citations (Scopus)

Abstract

Mouse and human dendritic cells (DCs) are composed of functionally specialized subsets, but precise interspecies correlation is currently incomplete. Here, we showed that murine lung and gut lamina propria CD11b+ DC populations were comprised of two subsets: FLT3- and IRF4-dependent CD24+CD64- DCs and contaminating CSF-1R-dependent CD24-CD64+ macrophages. Functionally, loss of CD24+CD11b+ DCs abrogated CD4+ Tcell-mediated interleukin-17 (IL-17) production in steady state and after Aspergillus fumigatus challenge. Human CD1c+ DCs, the equivalent of murine CD24+CD11b+ DCs, also expressed IRF4, secreted IL-23, and promoted T helper 17 cell responses. Our data revealed heterogeneity in the mouse CD11b+ DC compartment and identifed mucosal tissues IRF4-expressing DCs specialized ininstructing IL-17 responses in both mouse and human. The demonstration of mouse and human DC subsets specialized in driving IL-17 responses highlights the conservation of key immune functions across species and will facilitate the translation of mouse invivo findings to advance DC-based clinical therapies. •Mucosal CD11b+ DCs consist of CD24+CD64- DCs and CD24-CD64+ macrophages•Mucosal CD24+CD11b+ DCs are IRF4-dependent•IRF4-dependent CD24+CD11b+ DCs secrete IL-23α and control mucosal IL-17 responses•Human CD1c+CD11b+ DCs are functional homologs of murine CD24+CD11b+ DCs.

Original languageEnglish
Pages (from-to)970-983
Number of pages14
JournalImmunity
Volume38
Issue number5
DOIs
Publication statusPublished - 2013 May 23
Externally publishedYes

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Interleukin-17
Dendritic Cells
Transcription Factors
Cytokines
Interleukin-23
Mucous Membrane
Th17 Cells
Aspergillus fumigatus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

IRF4 Transcription Factor-Dependent CD11b+ Dendritic Cells in Human and Mouse Control Mucosal IL-17 Cytokine Responses. / Schlitzer, Andreas; McGovern, Naomi; Teo, Pearline; Zelante, Teresa; Atarashi, Koji; Low, Donovan; Ho, AdrianW S.; See, Peter; Shin, Amanda; Wasan, PavandipSingh; Hoeffel, Guillaume; Malleret, Benoit; Heiseke, Alexander; Chew, Samantha; Jardine, Laura; Purvis, HarrietA; Hilkens, CatharienM U.; Tam, John; Poidinger, Michael; Stanley, E. Richard; Krug, AnneB; Renia, Laurent; Sivasankar, Baalasubramanian; Ng, LaiGuan; Collin, Matthew; Ricciardi-Castagnoli, Paola; Honda, Kenya; Haniffa, Muzlifah; Ginhoux, Florent.

In: Immunity, Vol. 38, No. 5, 23.05.2013, p. 970-983.

Research output: Contribution to journalArticle

Schlitzer, A, McGovern, N, Teo, P, Zelante, T, Atarashi, K, Low, D, Ho, AS, See, P, Shin, A, Wasan, P, Hoeffel, G, Malleret, B, Heiseke, A, Chew, S, Jardine, L, Purvis, H, Hilkens, CU, Tam, J, Poidinger, M, Stanley, ER, Krug, A, Renia, L, Sivasankar, B, Ng, L, Collin, M, Ricciardi-Castagnoli, P, Honda, K, Haniffa, M & Ginhoux, F 2013, 'IRF4 Transcription Factor-Dependent CD11b+ Dendritic Cells in Human and Mouse Control Mucosal IL-17 Cytokine Responses', Immunity, vol. 38, no. 5, pp. 970-983. https://doi.org/10.1016/j.immuni.2013.04.011
Schlitzer, Andreas ; McGovern, Naomi ; Teo, Pearline ; Zelante, Teresa ; Atarashi, Koji ; Low, Donovan ; Ho, AdrianW S. ; See, Peter ; Shin, Amanda ; Wasan, PavandipSingh ; Hoeffel, Guillaume ; Malleret, Benoit ; Heiseke, Alexander ; Chew, Samantha ; Jardine, Laura ; Purvis, HarrietA ; Hilkens, CatharienM U. ; Tam, John ; Poidinger, Michael ; Stanley, E. Richard ; Krug, AnneB ; Renia, Laurent ; Sivasankar, Baalasubramanian ; Ng, LaiGuan ; Collin, Matthew ; Ricciardi-Castagnoli, Paola ; Honda, Kenya ; Haniffa, Muzlifah ; Ginhoux, Florent. / IRF4 Transcription Factor-Dependent CD11b+ Dendritic Cells in Human and Mouse Control Mucosal IL-17 Cytokine Responses. In: Immunity. 2013 ; Vol. 38, No. 5. pp. 970-983.
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AU - Teo, Pearline

AU - Zelante, Teresa

AU - Atarashi, Koji

AU - Low, Donovan

AU - Ho, AdrianW S.

AU - See, Peter

AU - Shin, Amanda

AU - Wasan, PavandipSingh

AU - Hoeffel, Guillaume

AU - Malleret, Benoit

AU - Heiseke, Alexander

AU - Chew, Samantha

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AU - Tam, John

AU - Poidinger, Michael

AU - Stanley, E. Richard

AU - Krug, AnneB

AU - Renia, Laurent

AU - Sivasankar, Baalasubramanian

AU - Ng, LaiGuan

AU - Collin, Matthew

AU - Ricciardi-Castagnoli, Paola

AU - Honda, Kenya

AU - Haniffa, Muzlifah

AU - Ginhoux, Florent

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