Iris damage is associated with elevated cytokine levels in aqueous humor

Naohiko Aketa, Takefumi Yamaguchi, Terumasa Suzuki, Kazunari Higa, Yukari Yagi-Yaguchi, Yoshiyuki Satake, Kazuo Tsubota, Jun Shimazaki

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20 Citations (Scopus)

Abstract

PURPOSE. To evaluate the association between iris damage and cytokine levels in the aqueous humor (AqH). METHODS. A total of 201 AqH samples from 201 consecutive patients (mean age 73.7 ± 10.6) were collected at the beginning of corneal transplantation or cataract surgery. Iris damage of each case was assessed from preoperative slit-lamp findings based on its severity. The subjects were classified into three groups: eyes without iris damage (126 eyes), eyes with mild iris damage (51 eyes), and eyes with severe iris damage (24 eyes). The levels of cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17a, interferon gamma-induced protein [IP]-10, monocyte chemotactic protein [MCP]-1, IFN-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble intercellular adhesion molecule [sICAM]-1, TNF-a, and granulocyte-macrophage colony-stimulating factor [GM-CSF]) in AqH were measured by multiplex beads immunoassay. RESULTS. The levels of aqueous protein, IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-17A, MCP-1, TNF-α, E-selectin, P-selectin, and sICAM-1 in eyes with mild and severe iris damage were higher than in those without iris damage (P < 0.033). Multivariate analyses of clinical factors revealed that iris damage was associated with the history of complicated glaucoma, and the number of previous intraocular surgeries. The levels of AqH IL-6, IL-8, IL-13, MIP-1α, TNF- α, and sICAM-1 were significantly elevated in eyes with mild and severe iris damage in phakic eyes, and the levels of AqH IL-8 and sICAM-1 were significantly elevated in eyes with severe iris damage in pseudophakic eyes, compared with the eyes without iris damage (P < 0.045). CONCLUSIONS. Iris damage was associated with the elevation in the levels of aqueous protein and cytokines.

Original languageEnglish
Pages (from-to)BIO42-BIO51
JournalInvestigative Ophthalmology and Visual Science
Volume58
Issue number6
DOIs
Publication statusPublished - 2017 May
Externally publishedYes

Keywords

  • Aqueous humor
  • Cytokine
  • Glaucoma
  • Immune privilege
  • Iris

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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  • Cite this

    Aketa, N., Yamaguchi, T., Suzuki, T., Higa, K., Yagi-Yaguchi, Y., Satake, Y., Tsubota, K., & Shimazaki, J. (2017). Iris damage is associated with elevated cytokine levels in aqueous humor. Investigative Ophthalmology and Visual Science, 58(6), BIO42-BIO51. https://doi.org/10.1167/iovs.17-21421