Abstract
Objective: The objective of the study was to clarify the relative contribution of islet number and islet size to α- and β-cell mass in humans. Research Design and Methods: We obtained the pancreas at autopsy from 72 Japanese adults with no history of diabetes or pancreatitis (aged 47 ± 12 years, body mass index 24.1 ± 5.0 kg/m2). Pancreatic sections were stained for insulin or glucagon, and fractional ±-cell area (%BCA) and β-cell area (%ACA) were measured. Islet number and islet size as well as ±-cell turnover were also quantified. Glycosylated hemoglobin measured within 1 year prior to death was obtained in 38 individuals. Results: There was considerable interindividual variation in islet density and mean islet size, with a significant negative correlation between the two (r = -0.25, P = .03). There were significant positive correlations between islet density and %BCA or %ACA (r = 0.63, P < .001, and r = 0.41, P = .001), whereas mean islet size correlated with neither of them. Islet density as well as %BCA, but not mean islet size, was negatively correlated with glycosylated hemoglobin (r=-0.37, P = .02, and r = -0.36, P = .03). Conclusion: The present study suggests that islet number rather than islet size is a major determinant of ±- and =-cell mass in humans. Interindividual difference in islet number may contribute to susceptibility to development of glucose intolerance.
| Original language | English |
|---|---|
| Pages (from-to) | 1733-1740 |
| Number of pages | 8 |
| Journal | Journal of Clinical Endocrinology and Metabolism |
| Volume | 99 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2014 |
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ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Endocrinology
- Biochemistry, medical
- Endocrinology, Diabetes and Metabolism
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Islet number rather than islet size is a major determinant of α- And β-Cell mass in humans. / Kou, Kinsei; Saisho, Yoshifumi; Sato, Seiji; Yamada, Taketo; Itoh, Hiroshi.
In: Journal of Clinical Endocrinology and Metabolism, Vol. 99, No. 5, 2014, p. 1733-1740.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Islet number rather than islet size is a major determinant of α- And β-Cell mass in humans
AU - Kou, Kinsei
AU - Saisho, Yoshifumi
AU - Sato, Seiji
AU - Yamada, Taketo
AU - Itoh, Hiroshi
PY - 2014
Y1 - 2014
N2 - Objective: The objective of the study was to clarify the relative contribution of islet number and islet size to α- and β-cell mass in humans. Research Design and Methods: We obtained the pancreas at autopsy from 72 Japanese adults with no history of diabetes or pancreatitis (aged 47 ± 12 years, body mass index 24.1 ± 5.0 kg/m2). Pancreatic sections were stained for insulin or glucagon, and fractional ±-cell area (%BCA) and β-cell area (%ACA) were measured. Islet number and islet size as well as ±-cell turnover were also quantified. Glycosylated hemoglobin measured within 1 year prior to death was obtained in 38 individuals. Results: There was considerable interindividual variation in islet density and mean islet size, with a significant negative correlation between the two (r = -0.25, P = .03). There were significant positive correlations between islet density and %BCA or %ACA (r = 0.63, P < .001, and r = 0.41, P = .001), whereas mean islet size correlated with neither of them. Islet density as well as %BCA, but not mean islet size, was negatively correlated with glycosylated hemoglobin (r=-0.37, P = .02, and r = -0.36, P = .03). Conclusion: The present study suggests that islet number rather than islet size is a major determinant of ±- and =-cell mass in humans. Interindividual difference in islet number may contribute to susceptibility to development of glucose intolerance.
AB - Objective: The objective of the study was to clarify the relative contribution of islet number and islet size to α- and β-cell mass in humans. Research Design and Methods: We obtained the pancreas at autopsy from 72 Japanese adults with no history of diabetes or pancreatitis (aged 47 ± 12 years, body mass index 24.1 ± 5.0 kg/m2). Pancreatic sections were stained for insulin or glucagon, and fractional ±-cell area (%BCA) and β-cell area (%ACA) were measured. Islet number and islet size as well as ±-cell turnover were also quantified. Glycosylated hemoglobin measured within 1 year prior to death was obtained in 38 individuals. Results: There was considerable interindividual variation in islet density and mean islet size, with a significant negative correlation between the two (r = -0.25, P = .03). There were significant positive correlations between islet density and %BCA or %ACA (r = 0.63, P < .001, and r = 0.41, P = .001), whereas mean islet size correlated with neither of them. Islet density as well as %BCA, but not mean islet size, was negatively correlated with glycosylated hemoglobin (r=-0.37, P = .02, and r = -0.36, P = .03). Conclusion: The present study suggests that islet number rather than islet size is a major determinant of ±- and =-cell mass in humans. Interindividual difference in islet number may contribute to susceptibility to development of glucose intolerance.
UR - http://www.scopus.com/inward/record.url?scp=84899905701&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899905701&partnerID=8YFLogxK
U2 - 10.1210/jc.2013-3731
DO - 10.1210/jc.2013-3731
M3 - Article
C2 - 24517149
AN - SCOPUS:84899905701
VL - 99
SP - 1733
EP - 1740
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 5
ER -