Isoform-specific alterations in cardiac and erythrocyte Na+,K+-ATPase activity induced by norepinephrine

Akiyasu Baba, Tsutomu Yoshikawa, Iwao Nakamura, Michikado Iwata, Yumiko Wainai, Satoshi Ogawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Myocardial Na+,K+-ATPase activities are decreased in congestive heart failure because of an increase in plasma norepinephrine levels, but it is difficult to monitor the activities in the clinical setting. Methods and Results: This study investigated whether erythrocyte Na+,K+-ATPase activity can reflect myocardial enzyme activity and whether isoform-specific alterations occur in the presence of catecholamine. Na+,K+-ATPase activity was measured by the colorimetric method by using the left ventricular myocardium and erythrocytes prepared from eight rabbits given norepinephrine for 7 days and from eight control rabbits that received saline. The protein levels of total catalytic subunit and α1- or α3- isoform of Na+,K+-ATPase were determined by Western blot analysis. Na+,K+-ATPase activity was lower in both myocardium and erythrocytes from norepinephrine-treated rabbits than control rabbits (P < .01 and P < .01, respectively). There was a close correlation in Na+,K+-ATPase activity between myocardium and erythrocytes (r = .963). Total catalytic subunit protein level was lower in myocardium from norepinephrine-treated rabbits than control rabbits, but the α1-isoform level was similar between the two groups. The α3-isoform level was lower in norepinephrine-treated rabbits than control rabbits. In erythrocytes, α1-isoform was lower in norepinephrine-treated rabbits than control rabbits. Conclusions: Na+,K+- ATPase activity in myocardium could be reflected in erythrocyte membrane, although there was a difference in isoform-specific regulation between the two.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalJournal of Cardiac Failure
Volume4
Issue number4
DOIs
Publication statusPublished - 1998 Dec

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Norepinephrine
Protein Isoforms
Erythrocytes
Rabbits
Myocardium
Catalytic Domain
sodium-translocating ATPase
Erythrocyte Membrane
Catecholamines
Proteins
Heart Failure
Western Blotting
Enzymes

Keywords

  • Catecholamine
  • Erythrocyte
  • Myocardium
  • Na,K-ATPase

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Isoform-specific alterations in cardiac and erythrocyte Na+,K+-ATPase activity induced by norepinephrine. / Baba, Akiyasu; Yoshikawa, Tsutomu; Nakamura, Iwao; Iwata, Michikado; Wainai, Yumiko; Ogawa, Satoshi.

In: Journal of Cardiac Failure, Vol. 4, No. 4, 12.1998, p. 333-341.

Research output: Contribution to journalArticle

Baba, Akiyasu ; Yoshikawa, Tsutomu ; Nakamura, Iwao ; Iwata, Michikado ; Wainai, Yumiko ; Ogawa, Satoshi. / Isoform-specific alterations in cardiac and erythrocyte Na+,K+-ATPase activity induced by norepinephrine. In: Journal of Cardiac Failure. 1998 ; Vol. 4, No. 4. pp. 333-341.
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