Isolation and characterization of a mouse SRY-related cDNA, mSox7

Koji Taniguchi; Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Susumu Kido; Sadakazu Aiso

doi:10.1016/S0167-4781(99)00047-0

Isolation and characterization of a mouse SRY-related cDNA, mSox7

Koji Taniguchi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Susumu Kido, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1445
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(99)00047-0
Publication status	Published - 1999 May 14
Externally published	Yes

Keywords

DNA binding
HMG box
Oocyte
SOX
SRY

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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title = "Isolation and characterization of a mouse SRY-related cDNA, mSox7",

abstract = "SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.",

keywords = "DNA binding, HMG box, Oocyte, SOX, SRY",

author = "Koji Taniguchi and Yoshiki Hiraoka and Motoyuki Ogawa and Yukinao Sakai and Susumu Kido and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in-Aid from the Ministry of Education, Science and Culture to S.A. and Y.H., by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

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doi = "10.1016/S0167-4781(99)00047-0",

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AU - Taniguchi, Koji

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Kido, Susumu

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in-Aid from the Ministry of Education, Science and Culture to S.A. and Y.H., by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

PY - 1999/5/14

Y1 - 1999/5/14

N2 - SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

AB - SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

KW - DNA binding

KW - HMG box

KW - Oocyte

KW - SOX

KW - SRY

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Isolation and characterization of a mouse SRY-related cDNA, mSox7

Abstract

Keywords

ASJC Scopus subject areas

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