Isolation and characterization of an auxin-binding protein gene from radish, and its expression in insect cells

Toyoaki Anai, Ryota Takai, Momo Miyata, Hidenobu Uchida, Seiji Kosemura, Shosuke Yamamura, Ryotaro Ishizaki, Koji Hasegawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Auxin-binding protein (ABP1) is a putative receptor for auxin in the plasma membrane. We isolated a full-length cDNA encoding ABP1 from radish by screening a cDNA library with its partial cDNA fragment generated by the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Radish abp1 mRNA was highly expressed in cotyledons, hooks and hypocotyls, and less so in roots of radish seedlings. The deduced amino acid sequence of radish abp1 consisted of conserved auxin-binding motifs, a signal peptide and ER-retention signal, and contained two potentially N-linked glycosylation signals. To analyze the biochemical properties of the radish abp1 product, this cDNA was expressed in insect cells by the baculovirus vector system. The result of tunicamycin-treatment showed that the signal peptide was cleaved and that the radish abp1 product was glycosylated at both target sites in insect cells.

Original languageEnglish
Pages (from-to)606-611
Number of pages6
JournalPhysiologia Plantarum
Volume101
Issue number3
DOIs
Publication statusPublished - 1997 Dec 1

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Keywords

  • Auxin-binding protein
  • Baculovirus vector
  • Insect cell
  • Radish
  • Raphanus sativus
  • cDNA cloning

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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