Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings

Toyoaki Anai, Naoko Kono, Seiji Kosemura, Shosuke Yamahura, Koji Hasegawa

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Abstract

A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the J'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promotem were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.

Original languageEnglish
Pages (from-to)329-333
Number of pages5
JournalMitochondrial DNA
Volume9
Issue number5-6
DOIs
Publication statusPublished - 1998 Dec 1

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Keywords

  • Raphanus sativus
  • SAW auxin-inducible gene
  • promoter region

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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