Isolation and characterization of anti-T-antigen single chain antibodies from a phage library

Ayano Matsumoto-Takasaki, Jinichiro Horie, Keiko Sakai, Yoshihiro Furui, Reiko Sato, Hiroko Kawakami, Kazunori Toma, Atsushi Takayanagi, Nobuyoshi Shimizu, Yoko Fujita-Yamaguchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

T-antigen (Galβ1-3GalNAc-Thr/Ser) also known as Thomsen-Friedenreich (TF) antigen is the core 1 structure of O-linked mucin type glycans. In normal epithelium, the disaccharide structure is masked by terminal carbohydrate moieties, but is uncovered in most primary and metastatic epithelial malignant tumors. For the purpose of establishing cancer diagnosis and therapeutics, anti-T-antigen antibodies were isolated from a phage library displaying human single chain antibodies. A strategy similar to the previously published method (Sakai et al. Biochemistry. 2007; 46:253-262) was used to screen T-antigen specific antibodies, except that a different type of glycolipid was used for panning and screening. Eleven phage clones were isolated and characterized by DNA sequencing and ELISA, which revealed 4 groups of clones with T-antigen binding activity. One single chain antibody (scFv) protein, derived from phage clone 1G11, was expressed in Escherichia coli and purified to near homogeneity by two column chromatographies. ELISA and surface plasmon resonance analyses revealed that the purified 1G11 scFv bound to the T-antigen moiety of the neoglycolipid used. This study not only demonstrated the validity of our previously introduced strategy employing the phage display technology in constructing human scFvs against various carbohydrate antigens, but also provided us with various scFv genes that can lead to future development of antibody-based therapeutics.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalBioScience Trends
Volume3
Issue number3
Publication statusPublished - 2009

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Single-Chain Antibodies
Bacteriophages
Viral Tumor Antigens
social isolation
biochemistry
Clone Cells
cancer
Antibodies
Enzyme-Linked Immunosorbent Assay
Carbohydrates
Column chromatography
Biochemistry
Surface Plasmon Resonance
Disaccharides
Glycolipids
Surface plasmon resonance
Mucins
DNA Sequence Analysis
Group
Escherichia coli

Keywords

  • Human antibodies
  • Neoglycolipids
  • Single chain antibodies
  • T-antigen
  • Thomsen-friedenreich (TF) antigen

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Health(social science)

Cite this

Matsumoto-Takasaki, A., Horie, J., Sakai, K., Furui, Y., Sato, R., Kawakami, H., ... Fujita-Yamaguchi, Y. (2009). Isolation and characterization of anti-T-antigen single chain antibodies from a phage library. BioScience Trends, 3(3), 87-95.

Isolation and characterization of anti-T-antigen single chain antibodies from a phage library. / Matsumoto-Takasaki, Ayano; Horie, Jinichiro; Sakai, Keiko; Furui, Yoshihiro; Sato, Reiko; Kawakami, Hiroko; Toma, Kazunori; Takayanagi, Atsushi; Shimizu, Nobuyoshi; Fujita-Yamaguchi, Yoko.

In: BioScience Trends, Vol. 3, No. 3, 2009, p. 87-95.

Research output: Contribution to journalArticle

Matsumoto-Takasaki, A, Horie, J, Sakai, K, Furui, Y, Sato, R, Kawakami, H, Toma, K, Takayanagi, A, Shimizu, N & Fujita-Yamaguchi, Y 2009, 'Isolation and characterization of anti-T-antigen single chain antibodies from a phage library', BioScience Trends, vol. 3, no. 3, pp. 87-95.
Matsumoto-Takasaki A, Horie J, Sakai K, Furui Y, Sato R, Kawakami H et al. Isolation and characterization of anti-T-antigen single chain antibodies from a phage library. BioScience Trends. 2009;3(3):87-95.
Matsumoto-Takasaki, Ayano ; Horie, Jinichiro ; Sakai, Keiko ; Furui, Yoshihiro ; Sato, Reiko ; Kawakami, Hiroko ; Toma, Kazunori ; Takayanagi, Atsushi ; Shimizu, Nobuyoshi ; Fujita-Yamaguchi, Yoko. / Isolation and characterization of anti-T-antigen single chain antibodies from a phage library. In: BioScience Trends. 2009 ; Vol. 3, No. 3. pp. 87-95.
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