Isolation and characterization of Xenopus laevis xSox-B1 cDNA

Yukinao Sakai, Yoshiki Hiraoka, Masahiro Konishi, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume346
Issue number1
DOIs
Publication statusPublished - 1997 Oct 1

Fingerprint

Xenopus laevis
Complementary DNA
Genes
sry Genes
Blastula
Messenger RNA
Polymerase chain reaction
Transcription
Anura
Reverse Transcription
Ovum
Ovary
Animals
Embryonic Structures
Tissue
Amino Acids
Polymerase Chain Reaction
Peptides
Proteins

Keywords

  • DNA binding
  • Embryogenesis
  • Nucleotide sequence
  • SOX
  • SRY (Xenopus laevis)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation and characterization of Xenopus laevis xSox-B1 cDNA. / Sakai, Yukinao; Hiraoka, Yoshiki; Konishi, Masahiro; Ogawa, Motoyuki; Aiso, Sadakazu.

In: Archives of Biochemistry and Biophysics, Vol. 346, No. 1, 01.10.1997, p. 1-6.

Research output: Contribution to journalArticle

Sakai, Yukinao ; Hiraoka, Yoshiki ; Konishi, Masahiro ; Ogawa, Motoyuki ; Aiso, Sadakazu. / Isolation and characterization of Xenopus laevis xSox-B1 cDNA. In: Archives of Biochemistry and Biophysics. 1997 ; Vol. 346, No. 1. pp. 1-6.
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