Isolation and characterization of Xenopus laevis xSox-B1 cDNA

Yukinao Sakai; Yoshiki Hiraoka; Masahiro Konishi; Motoyuki Ogawa; Sadakazu Aiso

doi:10.1006/abbi.1997.0283

Isolation and characterization of Xenopus laevis xSox-B1 cDNA

Yukinao Sakai, Yoshiki Hiraoka, Masahiro Konishi, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

Abstract

A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Archives of Biochemistry and Biophysics
Volume	346
Issue number	1
DOIs	https://doi.org/10.1006/abbi.1997.0283
Publication status	Published - 1997 Oct 1
Externally published	Yes

Keywords

DNA binding
Embryogenesis
Nucleotide sequence
SOX
SRY (Xenopus laevis)

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1006/abbi.1997.0283

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title = "Isolation and characterization of Xenopus laevis xSox-B1 cDNA",

abstract = "A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.",

keywords = "DNA binding, Embryogenesis, Nucleotide sequence, SOX, SRY (Xenopus laevis)",

author = "Yukinao Sakai and Yoshiki Hiraoka and Masahiro Konishi and Motoyuki Ogawa and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by grants from the Ministry of Education, Science, and Culture to S.A., Y.H., and O.M. and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Development Funds from Keio University to S.A. and Y.H.",

year = "1997",

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doi = "10.1006/abbi.1997.0283",

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AU - Sakai, Yukinao

AU - Hiraoka, Yoshiki

AU - Konishi, Masahiro

AU - Ogawa, Motoyuki

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by grants from the Ministry of Education, Science, and Culture to S.A., Y.H., and O.M. and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Development Funds from Keio University to S.A. and Y.H.

PY - 1997/10/1

Y1 - 1997/10/1

N2 - A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

AB - A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

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KW - Nucleotide sequence

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Isolation and characterization of Xenopus laevis xSox-B1 cDNA

Abstract

Keywords

ASJC Scopus subject areas

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