Isolation and functional analysis of a chk2 homologue from Entamoeba histolytica

Jun Iwashita, Yukita Sato, Seiki Kobayashi, Tsutomu Takeuchi, Tatsuya Abe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mammalian Chk2 is a Ser/Thr kinase required for cell-division arrest induced by DNA damage. We found six new kinase genes of Entamoeba histolytica by analysis in silico. One of the kinase genes was a homologue of human chk2 gene. The chk2 homologue gene (Eh chk2) was expected to encode 398 amino acids and showed nearly 50% homology to human Chk2 in amino acid sequence. Eh chk2 had a catalytic domain of Ser/Thr kinase and a fork head-associated (FHA) domain that is highly conserved among Chk2 homologues in vertebrates. To examine the biological functions of Eh chk2, we synthesized Eh chk2 mRNA in vitro and injected it into immature frog eggs (Xenopus laevis oocytes) as a model system of cell division. Eh chk2 markedly delayed the cell division of frog eggs by disrupting transition of G2 phase to M phase. Eh chk2 also inhibited the activation of p42 MAPK and Cdc2 kinase which are representative events induced by cell division. These results suggest that Eh chk2 gene should be a cell-division regulator in E. histolytica.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalParasitology International
Volume54
Issue number1
DOIs
Publication statusPublished - 2005 Mar

Fingerprint

Entamoeba histolytica
Cell Division
Phosphotransferases
Genes
Anura
Eggs
G2 Phase
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase Kinases
Xenopus laevis
Computer Simulation
DNA Damage
Oocytes
Vertebrates
Amino Acid Sequence
Catalytic Domain
Head
Amino Acids
Messenger RNA

Keywords

  • Cell division
  • Chk2
  • Entamoeba histolytica
  • Kinase

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Isolation and functional analysis of a chk2 homologue from Entamoeba histolytica. / Iwashita, Jun; Sato, Yukita; Kobayashi, Seiki; Takeuchi, Tsutomu; Abe, Tatsuya.

In: Parasitology International, Vol. 54, No. 1, 03.2005, p. 21-27.

Research output: Contribution to journalArticle

Iwashita, Jun ; Sato, Yukita ; Kobayashi, Seiki ; Takeuchi, Tsutomu ; Abe, Tatsuya. / Isolation and functional analysis of a chk2 homologue from Entamoeba histolytica. In: Parasitology International. 2005 ; Vol. 54, No. 1. pp. 21-27.
@article{5fdf7b400f4d4bfc931017c0ed35001c,
title = "Isolation and functional analysis of a chk2 homologue from Entamoeba histolytica",
abstract = "Mammalian Chk2 is a Ser/Thr kinase required for cell-division arrest induced by DNA damage. We found six new kinase genes of Entamoeba histolytica by analysis in silico. One of the kinase genes was a homologue of human chk2 gene. The chk2 homologue gene (Eh chk2) was expected to encode 398 amino acids and showed nearly 50{\%} homology to human Chk2 in amino acid sequence. Eh chk2 had a catalytic domain of Ser/Thr kinase and a fork head-associated (FHA) domain that is highly conserved among Chk2 homologues in vertebrates. To examine the biological functions of Eh chk2, we synthesized Eh chk2 mRNA in vitro and injected it into immature frog eggs (Xenopus laevis oocytes) as a model system of cell division. Eh chk2 markedly delayed the cell division of frog eggs by disrupting transition of G2 phase to M phase. Eh chk2 also inhibited the activation of p42 MAPK and Cdc2 kinase which are representative events induced by cell division. These results suggest that Eh chk2 gene should be a cell-division regulator in E. histolytica.",
keywords = "Cell division, Chk2, Entamoeba histolytica, Kinase",
author = "Jun Iwashita and Yukita Sato and Seiki Kobayashi and Tsutomu Takeuchi and Tatsuya Abe",
year = "2005",
month = "3",
doi = "10.1016/j.parint.2004.08.002",
language = "English",
volume = "54",
pages = "21--27",
journal = "Parasitology International",
issn = "1383-5769",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Isolation and functional analysis of a chk2 homologue from Entamoeba histolytica

AU - Iwashita, Jun

AU - Sato, Yukita

AU - Kobayashi, Seiki

AU - Takeuchi, Tsutomu

AU - Abe, Tatsuya

PY - 2005/3

Y1 - 2005/3

N2 - Mammalian Chk2 is a Ser/Thr kinase required for cell-division arrest induced by DNA damage. We found six new kinase genes of Entamoeba histolytica by analysis in silico. One of the kinase genes was a homologue of human chk2 gene. The chk2 homologue gene (Eh chk2) was expected to encode 398 amino acids and showed nearly 50% homology to human Chk2 in amino acid sequence. Eh chk2 had a catalytic domain of Ser/Thr kinase and a fork head-associated (FHA) domain that is highly conserved among Chk2 homologues in vertebrates. To examine the biological functions of Eh chk2, we synthesized Eh chk2 mRNA in vitro and injected it into immature frog eggs (Xenopus laevis oocytes) as a model system of cell division. Eh chk2 markedly delayed the cell division of frog eggs by disrupting transition of G2 phase to M phase. Eh chk2 also inhibited the activation of p42 MAPK and Cdc2 kinase which are representative events induced by cell division. These results suggest that Eh chk2 gene should be a cell-division regulator in E. histolytica.

AB - Mammalian Chk2 is a Ser/Thr kinase required for cell-division arrest induced by DNA damage. We found six new kinase genes of Entamoeba histolytica by analysis in silico. One of the kinase genes was a homologue of human chk2 gene. The chk2 homologue gene (Eh chk2) was expected to encode 398 amino acids and showed nearly 50% homology to human Chk2 in amino acid sequence. Eh chk2 had a catalytic domain of Ser/Thr kinase and a fork head-associated (FHA) domain that is highly conserved among Chk2 homologues in vertebrates. To examine the biological functions of Eh chk2, we synthesized Eh chk2 mRNA in vitro and injected it into immature frog eggs (Xenopus laevis oocytes) as a model system of cell division. Eh chk2 markedly delayed the cell division of frog eggs by disrupting transition of G2 phase to M phase. Eh chk2 also inhibited the activation of p42 MAPK and Cdc2 kinase which are representative events induced by cell division. These results suggest that Eh chk2 gene should be a cell-division regulator in E. histolytica.

KW - Cell division

KW - Chk2

KW - Entamoeba histolytica

KW - Kinase

UR - http://www.scopus.com/inward/record.url?scp=13844297311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844297311&partnerID=8YFLogxK

U2 - 10.1016/j.parint.2004.08.002

DO - 10.1016/j.parint.2004.08.002

M3 - Article

VL - 54

SP - 21

EP - 27

JO - Parasitology International

JF - Parasitology International

SN - 1383-5769

IS - 1

ER -