Isolation and identification of a phytotoxic substance in kiwifruit leaves

S. Okada, Arihiro Iwasaki, I. Kataoka, Kiyotake Suenaga, H. Kato-Noguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The yield of kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson] often decreases after 20 years of cultivation. Replanted young kiwifruit plants cannot grow well in fields where aged trees were previously cultivated. Phytotoxicity is considered one possible reason for this problem. However, allelopathic or phytotoxic substances from kiwifruit have not been reported. This study aimed to isolate and identify potentially allelopathic active substances in kiwifruit leaves. Extracts from kiwifruit leaves inhibited the growth of cress, suggesting that kiwifruit leaf extracts may contain growth-inhibitory substances. The extracts were then purified using a silica-gel column, Sephadex LH-20 column, ODS cartridge, and HPLC. The inhibitory activity of all fractions obtained in each purification step was determined by the cress bioassay. Quercitrin was finally isolated and identified as a growth-inhibitory substance. This substance inhibited cress root growth by more than 50% of control at 3 mM. The aglycone of quercitrin, quercetin, did not significantly inhibit cress growth. Quercitrin may work as an allelopathic substance and cause the kiwifruit replant problem. Further study is in progress to investigate the influence of kiwifruit leaf extracts and these flavonoids on kiwifruit seedlings.

Original languageEnglish
Title of host publication9th International Symposium on Kiwifruit
EditorsM.D. Antunes, P.P. Gallego
PublisherInternational Society for Horticultural Science
Pages207-212
Number of pages6
ISBN (Electronic)9789462612150
Publication statusPublished - 2018 Oct 31

Publication series

NameActa Horticulturae
Volume1218
ISSN (Print)0567-7572
ISSN (Electronic)2406-6168

Fingerprint

kiwifruit
quercitrin
leaves
leaf extracts
Actinidia deliciosa
allelochemicals
extracts
silica gel
phytotoxicity
quercetin
root growth
flavonoids
bioassays
seedlings

Keywords

  • Actinidia deliciosa
  • Allelopathy
  • Growth inhibitory substance
  • Quercitrin
  • Replant problem

ASJC Scopus subject areas

  • Horticulture

Cite this

Okada, S., Iwasaki, A., Kataoka, I., Suenaga, K., & Kato-Noguchi, H. (2018). Isolation and identification of a phytotoxic substance in kiwifruit leaves. In M. D. Antunes, & P. P. Gallego (Eds.), 9th International Symposium on Kiwifruit (pp. 207-212). (Acta Horticulturae; Vol. 1218). International Society for Horticultural Science.

Isolation and identification of a phytotoxic substance in kiwifruit leaves. / Okada, S.; Iwasaki, Arihiro; Kataoka, I.; Suenaga, Kiyotake; Kato-Noguchi, H.

9th International Symposium on Kiwifruit. ed. / M.D. Antunes; P.P. Gallego. International Society for Horticultural Science, 2018. p. 207-212 (Acta Horticulturae; Vol. 1218).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Okada, S, Iwasaki, A, Kataoka, I, Suenaga, K & Kato-Noguchi, H 2018, Isolation and identification of a phytotoxic substance in kiwifruit leaves. in MD Antunes & PP Gallego (eds), 9th International Symposium on Kiwifruit. Acta Horticulturae, vol. 1218, International Society for Horticultural Science, pp. 207-212.
Okada S, Iwasaki A, Kataoka I, Suenaga K, Kato-Noguchi H. Isolation and identification of a phytotoxic substance in kiwifruit leaves. In Antunes MD, Gallego PP, editors, 9th International Symposium on Kiwifruit. International Society for Horticultural Science. 2018. p. 207-212. (Acta Horticulturae).
Okada, S. ; Iwasaki, Arihiro ; Kataoka, I. ; Suenaga, Kiyotake ; Kato-Noguchi, H. / Isolation and identification of a phytotoxic substance in kiwifruit leaves. 9th International Symposium on Kiwifruit. editor / M.D. Antunes ; P.P. Gallego. International Society for Horticultural Science, 2018. pp. 207-212 (Acta Horticulturae).
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abstract = "The yield of kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson] often decreases after 20 years of cultivation. Replanted young kiwifruit plants cannot grow well in fields where aged trees were previously cultivated. Phytotoxicity is considered one possible reason for this problem. However, allelopathic or phytotoxic substances from kiwifruit have not been reported. This study aimed to isolate and identify potentially allelopathic active substances in kiwifruit leaves. Extracts from kiwifruit leaves inhibited the growth of cress, suggesting that kiwifruit leaf extracts may contain growth-inhibitory substances. The extracts were then purified using a silica-gel column, Sephadex LH-20 column, ODS cartridge, and HPLC. The inhibitory activity of all fractions obtained in each purification step was determined by the cress bioassay. Quercitrin was finally isolated and identified as a growth-inhibitory substance. This substance inhibited cress root growth by more than 50{\%} of control at 3 mM. The aglycone of quercitrin, quercetin, did not significantly inhibit cress growth. Quercitrin may work as an allelopathic substance and cause the kiwifruit replant problem. Further study is in progress to investigate the influence of kiwifruit leaf extracts and these flavonoids on kiwifruit seedlings.",
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