Isolation and identification of a plant growth inhibitor from Tinospora tuberculata Beumee

Hisashi Kato-Noguchi, Piyatida Pukclai, Osamu Ohno, Kiyotake Suenaga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Tinospora tuberculata Beumee has been used widely as a folk medicine and several bioactive compounds have been isolated. However, no herbicidal compound has been reported in this species. Therefore, we investigated the presence of phytotoxins in T. tuberculata. The aqueous methanol extracts of T. tuberculata inhibited the growth of roots and shoots of cress (Lepidum sativum L.), lettuce (Lactuca sativa L.), timothy (Phleum pratense L.) and barnyard grass (Echinochloa crus-galli (L.) Beauv.). The extract was then purified by several chromatographic runs with monitoring the inhibitory activity and the main phytotoxic substance was isolated. The chemical structure of the compound was determined by spectral data as syringin (4-[(1E)-3-Hydroxyprop-1-en-1-yl]-2,6-dimethoxyphenyl β-d-glucopyranoside). It inhibited the root and shoot growth of all test plant species at concentrations >10 μM. The concentrations required for 50 % inhibition of root and shoot growth of cress and lettuce ranged from 78.2 to 412 μM, and that of timothy and barnyard grass renged from 9.8 to 73.2 μM. Effectiveness of syringin on monocotyledonous (timothy and barnyard grass) plants was greater than that on dicotyledonous (cress and lettuce) plants. These results suggest that syringin may contribute to the allelopathic effect caused by the T. tuberculata extract.

Original languageEnglish
Pages (from-to)1621-1626
Number of pages6
JournalActa Physiologiae Plantarum
Volume36
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

Tinospora
Echinochloa
Lettuce
Growth Inhibitors
Echinochloa crus-galli
Phleum
Brassicaceae
Phleum pratense
plant growth
lettuce
shoots
extracts
Growth
phytotoxins
Lactuca sativa
Traditional Medicine
traditional medicine
chemical structure
spectral analysis
Methanol

Keywords

  • Allelopathy
  • Bioactive compound
  • Menispermaceae
  • Phytotoxin
  • Syringin
  • Tinospora tuberculata

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Isolation and identification of a plant growth inhibitor from Tinospora tuberculata Beumee. / Kato-Noguchi, Hisashi; Pukclai, Piyatida; Ohno, Osamu; Suenaga, Kiyotake.

In: Acta Physiologiae Plantarum, Vol. 36, No. 7, 2014, p. 1621-1626.

Research output: Contribution to journalArticle

Kato-Noguchi, Hisashi ; Pukclai, Piyatida ; Ohno, Osamu ; Suenaga, Kiyotake. / Isolation and identification of a plant growth inhibitor from Tinospora tuberculata Beumee. In: Acta Physiologiae Plantarum. 2014 ; Vol. 36, No. 7. pp. 1621-1626.
@article{c7848974e5964c40850d58490e8856ec,
title = "Isolation and identification of a plant growth inhibitor from Tinospora tuberculata Beumee",
abstract = "Tinospora tuberculata Beumee has been used widely as a folk medicine and several bioactive compounds have been isolated. However, no herbicidal compound has been reported in this species. Therefore, we investigated the presence of phytotoxins in T. tuberculata. The aqueous methanol extracts of T. tuberculata inhibited the growth of roots and shoots of cress (Lepidum sativum L.), lettuce (Lactuca sativa L.), timothy (Phleum pratense L.) and barnyard grass (Echinochloa crus-galli (L.) Beauv.). The extract was then purified by several chromatographic runs with monitoring the inhibitory activity and the main phytotoxic substance was isolated. The chemical structure of the compound was determined by spectral data as syringin (4-[(1E)-3-Hydroxyprop-1-en-1-yl]-2,6-dimethoxyphenyl β-d-glucopyranoside). It inhibited the root and shoot growth of all test plant species at concentrations >10 μM. The concentrations required for 50 {\%} inhibition of root and shoot growth of cress and lettuce ranged from 78.2 to 412 μM, and that of timothy and barnyard grass renged from 9.8 to 73.2 μM. Effectiveness of syringin on monocotyledonous (timothy and barnyard grass) plants was greater than that on dicotyledonous (cress and lettuce) plants. These results suggest that syringin may contribute to the allelopathic effect caused by the T. tuberculata extract.",
keywords = "Allelopathy, Bioactive compound, Menispermaceae, Phytotoxin, Syringin, Tinospora tuberculata",
author = "Hisashi Kato-Noguchi and Piyatida Pukclai and Osamu Ohno and Kiyotake Suenaga",
year = "2014",
doi = "10.1007/s11738-014-1537-5",
language = "English",
volume = "36",
pages = "1621--1626",
journal = "Acta Physiologiae Plantarum",
issn = "0137-5881",
publisher = "Polish Academy of Sciences Publishing House",
number = "7",

}

TY - JOUR

T1 - Isolation and identification of a plant growth inhibitor from Tinospora tuberculata Beumee

AU - Kato-Noguchi, Hisashi

AU - Pukclai, Piyatida

AU - Ohno, Osamu

AU - Suenaga, Kiyotake

PY - 2014

Y1 - 2014

N2 - Tinospora tuberculata Beumee has been used widely as a folk medicine and several bioactive compounds have been isolated. However, no herbicidal compound has been reported in this species. Therefore, we investigated the presence of phytotoxins in T. tuberculata. The aqueous methanol extracts of T. tuberculata inhibited the growth of roots and shoots of cress (Lepidum sativum L.), lettuce (Lactuca sativa L.), timothy (Phleum pratense L.) and barnyard grass (Echinochloa crus-galli (L.) Beauv.). The extract was then purified by several chromatographic runs with monitoring the inhibitory activity and the main phytotoxic substance was isolated. The chemical structure of the compound was determined by spectral data as syringin (4-[(1E)-3-Hydroxyprop-1-en-1-yl]-2,6-dimethoxyphenyl β-d-glucopyranoside). It inhibited the root and shoot growth of all test plant species at concentrations >10 μM. The concentrations required for 50 % inhibition of root and shoot growth of cress and lettuce ranged from 78.2 to 412 μM, and that of timothy and barnyard grass renged from 9.8 to 73.2 μM. Effectiveness of syringin on monocotyledonous (timothy and barnyard grass) plants was greater than that on dicotyledonous (cress and lettuce) plants. These results suggest that syringin may contribute to the allelopathic effect caused by the T. tuberculata extract.

AB - Tinospora tuberculata Beumee has been used widely as a folk medicine and several bioactive compounds have been isolated. However, no herbicidal compound has been reported in this species. Therefore, we investigated the presence of phytotoxins in T. tuberculata. The aqueous methanol extracts of T. tuberculata inhibited the growth of roots and shoots of cress (Lepidum sativum L.), lettuce (Lactuca sativa L.), timothy (Phleum pratense L.) and barnyard grass (Echinochloa crus-galli (L.) Beauv.). The extract was then purified by several chromatographic runs with monitoring the inhibitory activity and the main phytotoxic substance was isolated. The chemical structure of the compound was determined by spectral data as syringin (4-[(1E)-3-Hydroxyprop-1-en-1-yl]-2,6-dimethoxyphenyl β-d-glucopyranoside). It inhibited the root and shoot growth of all test plant species at concentrations >10 μM. The concentrations required for 50 % inhibition of root and shoot growth of cress and lettuce ranged from 78.2 to 412 μM, and that of timothy and barnyard grass renged from 9.8 to 73.2 μM. Effectiveness of syringin on monocotyledonous (timothy and barnyard grass) plants was greater than that on dicotyledonous (cress and lettuce) plants. These results suggest that syringin may contribute to the allelopathic effect caused by the T. tuberculata extract.

KW - Allelopathy

KW - Bioactive compound

KW - Menispermaceae

KW - Phytotoxin

KW - Syringin

KW - Tinospora tuberculata

UR - http://www.scopus.com/inward/record.url?scp=84903307683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903307683&partnerID=8YFLogxK

U2 - 10.1007/s11738-014-1537-5

DO - 10.1007/s11738-014-1537-5

M3 - Article

AN - SCOPUS:84903307683

VL - 36

SP - 1621

EP - 1626

JO - Acta Physiologiae Plantarum

JF - Acta Physiologiae Plantarum

SN - 0137-5881

IS - 7

ER -