Isolation and identification of an allelopathic substance from duckweed (Lemna minor L.)

T. T N Bich, O. Ohno, Kiyotake Suenaga, H. Kato-Noguchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An aqueous methanol extract of duckweed (Lemna minor L.) inhibited the growth of cress (Lepidium sativum L.). The extract was partitioned by silica gel, Diaion HP20, Sephadex LH-20 and Diaion HP20SS columns, C18 Sep-Pak cartridges and reverse phase HPLC to isolate phytotoxic substances. The (3R)-(-)-3-hydroxy-β- ionone was identified as the active ingredient in the extract by its spectral data. (3R)-(-)-3-hydroxy-β-ionone inhibited growth of cress and Italian ryegrass seedlings at ≥0.1 and ≥5 μM, respectively. (3R)-(-)-3-Hydroxy-β-ionone inhibited 50% of cress roots and shoots growth with 0.1 μM and 50% of Italian ryegrass root and shoot growth at 2.4 and 3.4 μM, respectively. The presence of (3R)-(-)-3-hydroxy-β-ionone in duckweed and its growth inhibitory activity suggested that it may contribute to the allelopathic potential of duckweed.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalAllelopathy Journal
Volume32
Issue number2
Publication statusPublished - 2013

Fingerprint

Lemna minor
norisoprenoids
allelochemicals
Lolium multiflorum
extracts
Lepidium sativum
shoots
silica gel
active ingredients
spectral analysis
methanol
seedlings
water

Keywords

  • 3-hydroxy-β-ionone
  • Allelopathy
  • Aquatic plant
  • Duckweed
  • Eco-agriculture
  • Growth inhibitor
  • Ionone
  • Italian ryegrass
  • Natural resource
  • Weed management

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Isolation and identification of an allelopathic substance from duckweed (Lemna minor L.). / Bich, T. T N; Ohno, O.; Suenaga, Kiyotake; Kato-Noguchi, H.

In: Allelopathy Journal, Vol. 32, No. 2, 2013, p. 213-222.

Research output: Contribution to journalArticle

Bich, T. T N ; Ohno, O. ; Suenaga, Kiyotake ; Kato-Noguchi, H. / Isolation and identification of an allelopathic substance from duckweed (Lemna minor L.). In: Allelopathy Journal. 2013 ; Vol. 32, No. 2. pp. 213-222.
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