Isolation and identification of an allelopathic substance from hibiscus sabdariffa

Prapaipit Suwitchayanon, Piyatida Pukclai, Osamu Ohno, Kiyotake Suenaga, Hisashi Kato-Noguchi

Research output: Contribution to journalArticle

2 Citations (Scopus)


In this study, an allelopathic substance was isolated from an aqueous methanol extract of Hibiscus sabdariffa L. by column chromatography and reverse phase HPLC. The chemical structure of the substance was determined by 1H NMR spectroscopy and mass spectrometry as trimethyl allo-hydroxycitrate. Trimethyl allo-hydroxycitrate inhibited the growth of cress hypocotyls and roots at concentrations greater than 10 mM. The concentrations required for 50% growth inhibition of the hypocotyls and roots of cress were 20.3 and 14.4 mM, respectively. The inhibitory activity of trimethyl allo-hydroxycitrate suggests that the substance may act as an allelopathic substance of H. sabdariffa.

Original languageEnglish
Pages (from-to)765-766
Number of pages2
JournalNatural Product Communications
Issue number5
Publication statusPublished - 2015



  • Allelopathic substance
  • Hibiscus sabdariffa
  • Phytotoxic activity
  • Trimethyl allo-hydroxycitrate

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Complementary and alternative medicine
  • Plant Science

Cite this

Suwitchayanon, P., Pukclai, P., Ohno, O., Suenaga, K., & Kato-Noguchi, H. (2015). Isolation and identification of an allelopathic substance from hibiscus sabdariffa. Natural Product Communications, 10(5), 765-766.