Isolation and identification of canine plasma components suspected as uremic toxins.

M. Kawamura, F. Ohashi, Y. Nagata, N. Takai, H. Motoie, R. Nishimura, Yoshiko Itoh, A. Takeuchi

Research output: Contribution to journalArticle

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Abstract

Suspected uremic substances contained in four fractions, which had been selected as the suspected canine uremic peaks in the previous study, were isolated by two stages of preparative liquid chromatography (PLC) from plasma of uremic dogs treated with the ligation of the ureter, and then their physicochemical properties were examined. The primary separation of the suspected uremic peaks were performed with the same anion exchange resin as used in the analytical HPLC in the previous study. Analytical reverse phase HPLC showed that three of 4 suspected uremic peaks almost consisted of single substances, but the other contained several substances. Main subfractions of these peaks were successfully isolated by the secondary stage reverse phase PLC. By means of thin layer chromatography, ultraviolet absorption spectrometry and proton-nuclear magnetic resonance spectroscopy, components of 4 main peaks were confirmed to be small molecules such as a pyridine derivative, uric acid, hippuric acid and kynurenic acid, respectively.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalThe Journal of veterinary medical science / the Japanese Society of Veterinary Science
Volume55
Issue number2
Publication statusPublished - 1993 Apr

Fingerprint

Canidae
toxins
high performance liquid chromatography
High Pressure Liquid Chromatography
hippuric acid
Anion Exchange Resins
Kynurenic Acid
anion exchange resins
ureter
dogs
reversed-phase liquid chromatography
pyridines
Reverse-Phase Chromatography
uric acid
Ureter
Thin Layer Chromatography
Uric Acid
thin layer chromatography
Liquid Chromatography
liquid chromatography

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

Cite this

Kawamura, M., Ohashi, F., Nagata, Y., Takai, N., Motoie, H., Nishimura, R., ... Takeuchi, A. (1993). Isolation and identification of canine plasma components suspected as uremic toxins. The Journal of veterinary medical science / the Japanese Society of Veterinary Science, 55(2), 265-270.

Isolation and identification of canine plasma components suspected as uremic toxins. / Kawamura, M.; Ohashi, F.; Nagata, Y.; Takai, N.; Motoie, H.; Nishimura, R.; Itoh, Yoshiko; Takeuchi, A.

In: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 55, No. 2, 04.1993, p. 265-270.

Research output: Contribution to journalArticle

Kawamura, M, Ohashi, F, Nagata, Y, Takai, N, Motoie, H, Nishimura, R, Itoh, Y & Takeuchi, A 1993, 'Isolation and identification of canine plasma components suspected as uremic toxins.', The Journal of veterinary medical science / the Japanese Society of Veterinary Science, vol. 55, no. 2, pp. 265-270.
Kawamura, M. ; Ohashi, F. ; Nagata, Y. ; Takai, N. ; Motoie, H. ; Nishimura, R. ; Itoh, Yoshiko ; Takeuchi, A. / Isolation and identification of canine plasma components suspected as uremic toxins. In: The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1993 ; Vol. 55, No. 2. pp. 265-270.
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