Isolation and identification of hematopoietic stem cell-stimulating substances from kampo (Japanese herbal) medicine, Juzen-Taiho-To

Hiroko Hisha, Haruki Yamada, Masumi H. Sakurai, Hiroaki Kiyohara, Yongan Li, Cheng Ze Yu, Norito Takemoto, Hideki Kawamura, Katsunori Yamaura, Seiichi Shinohara, Yasuhiro Komatsu, Masaki Aburada, Susumu Ikehara

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

We have previously found that T J-48 has the capacity to accelerate recovery from hematopoietic injury induced by radiation and the anti-cancer drug mitomycin C (MMC). The effects are found to be due to its stimulation of spleen colony-forming unit (CFU-S) counts on day 14. In the present study, we attempt to isolate and purify the active components in T J-48 extracts using a new in vitro hematopoietic stem cell (HSC) assay method. n-Hexane extract from TJ-48 shows a significant stimulatory activity. The extract is further fractionated by silica gel chromatography and HPLC in order to identify its active components. 1H-NMR and GC-El-MS indicate that the active fraction is composed of free fatty acids (oleic acid and linolenic acid). When 27 kinds of free fatty acids (commercially available) are tested using the HSC proliferating assay, oleic acid, elaidic acid, and linolenic acid are found to have potent activity. The administration of oleic acid to MMC-treated mice enhances CFU-S counts on days 8 and 14 to twice the control group. These findings strongly suggest that fatty acids contained in TJ-48 actively promote the proliferation of HSCs. Although many mechanisms seem to be involved in the stimulation of HSC proliferation, we speculate that at least one of the signals is mediated by stromal cells, rather than any direct interaction with the HSCs.

Original languageEnglish
Pages (from-to)1022-1030
Number of pages9
JournalBlood
Volume90
Issue number3
DOIs
Publication statusPublished - 1997 Aug 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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