Isolation and identification of potent allelopathic substances in rattail fescue

Hisashi Kato-Noguchi, Madoka Yamamoto, Kazuya Tamura, Toshiaki Teruya, Kiyotake Suenaga, Yoshiharu Fujii

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aqueous methanol extracts of rattail fescue (Vulpia myuros) inhibited the growth of roots and shoots of cress (Lepidium sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), timothy (Phleum pratense), Digitaria sanguinalis and Lolium multiflorum. Increasing the extract concentration increased the inhibition, suggesting that rattail fescue may have growth inhibitory substances and possess allelopathic potential. The aqueous methanol extract of rattail fescue was purified and two main inhibitory substances were isolated and identified by spectral data as (-)-3-hydroxy-β-ionone and (+)-3-oxo-α-ionol. Both substances inhibited root and shoot growth of cress at concentrations greater than 0.3 μM. The concentrations required for 50% growth inhibition on root and shoot growth of cress, lettuce, alfalfa, timothy, D. sanguinalis and L. multiflorum were 2.7-19.7 μM for (-)-3-hydroxy-β-ionone, and 2.1-34.5 μM for (+)-3-oxo-α-ionol. The concentration of (-)-3-hydroxy-β-ionone and (+)-3-oxo-α-ionol, respectively, in rattail fescue was 7.8 and 3.7 μg g-1 fresh weight. Considering the endogenous level and the inhibitory activity, (-)-3-hydroxy-β-ionone and (+)-3-oxo-α-ionol may work as allelopathic substances in rattail fescue through the growth inhibition of neighboring plant species.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalPlant Growth Regulation
Volume60
Issue number2
DOIs
Publication statusPublished - 2010 Mar 1

Keywords

  • Allelopathy
  • Biological activity
  • Growth inhibitor
  • Ionone
  • Rattail fescue
  • Vulpia

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Isolation and identification of potent allelopathic substances in rattail fescue'. Together they form a unique fingerprint.

  • Cite this