Isolation and identification of two potential phytotoxic substances from the aquatic fern Marsilea crenata

Md Shafiqul Islam, Arihiro Iwasaki, Kiyotake Suenaga, Hisashi Kato-Noguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Organic agriculture emphasized using of biologically originated herbicides and phytotoxic substances are being considered as a replacement to chemical herbicides. Marsilea crenata is an aquatic perennial fern distributed in the South-East Asian countries and is well known for various biological properties. However, to date, there has been no report that addresses the phytotoxicity of Marsilea crenata. Therefore, we explored phytotoxic properties and phytotoxic substances from Marsilea crenata. An aqueous methanol extracts of Marsilea crenata showed inhibition on the seedling growth of cress, lettuce, alfalfa, barnyard grass, Italian ryegrass, and foxtail fescue. Inhibition increased with increasing extract concentration. The extract was purified by several chromatographic steps and two phytotoxic substances were isolated and identified by spectroscopic analysis as loliolide and isololiolide. At the concentration of 30 μM, loliolide and isololiolide inhibited seedling growth of cress and barnyard grass by 41.3 to 51.1%, and 58.15 to 87.5% of control seedlings, respectively. The concentrations required for 50% inhibition of cress and barnyard grass seedlings ranged from 32.1 to 128.5 μM for loliolide, 37.0 to 176.2 μM for isololiolide. These results suggest these compounds may be responsible for phytotoxic effects of Marsilea crenata extract and could be an important part of organic agriculture.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalJournal of Plant Biology
Volume60
Issue number1
DOIs
Publication statusPublished - 2017 Feb 1

Fingerprint

Marsilea
ferns and fern allies
Echinochloa crus-galli
extracts
organic production
seedling growth
herbicides
seedlings
Festuca
Lolium multiflorum
phytotoxicity
South East Asia
lettuce
alfalfa
methanol

Keywords

  • Aqueous methanol extract
  • Isololiolide
  • Loliolide
  • Marsilea crenata
  • Organic agriculture

ASJC Scopus subject areas

  • Plant Science

Cite this

Isolation and identification of two potential phytotoxic substances from the aquatic fern Marsilea crenata. / Islam, Md Shafiqul; Iwasaki, Arihiro; Suenaga, Kiyotake; Kato-Noguchi, Hisashi.

In: Journal of Plant Biology, Vol. 60, No. 1, 01.02.2017, p. 75-81.

Research output: Contribution to journalArticle

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