Isolation and in vitro expansion of human colonic stem cells

Peter Jung, Toshiro Sato, Anna Merlos-Suárez, Francisco M. Barriga, Mar Iglesias, David Rossell, Herbert Auer, Mercedes Gallardo, Maria A. Blasco, Elena Sancho, Hans Clevers, Eduard Batlle

Research output: Contribution to journalArticle

349 Citations (Scopus)

Abstract

Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.

Original languageEnglish
Pages (from-to)1225-1227
Number of pages3
JournalNature Medicine
Volume17
Issue number10
DOIs
Publication statusPublished - 2011 Oct

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Eph Family Receptors
Stem cells
Stem Cells
Stem Cell Niche
Biopsy
Telomere
Purification
Colon
Mucous Membrane
Epithelium
Genes
Epithelial Cells
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Jung, P., Sato, T., Merlos-Suárez, A., Barriga, F. M., Iglesias, M., Rossell, D., ... Batlle, E. (2011). Isolation and in vitro expansion of human colonic stem cells. Nature Medicine, 17(10), 1225-1227. https://doi.org/10.1038/nm.2470

Isolation and in vitro expansion of human colonic stem cells. / Jung, Peter; Sato, Toshiro; Merlos-Suárez, Anna; Barriga, Francisco M.; Iglesias, Mar; Rossell, David; Auer, Herbert; Gallardo, Mercedes; Blasco, Maria A.; Sancho, Elena; Clevers, Hans; Batlle, Eduard.

In: Nature Medicine, Vol. 17, No. 10, 10.2011, p. 1225-1227.

Research output: Contribution to journalArticle

Jung, P, Sato, T, Merlos-Suárez, A, Barriga, FM, Iglesias, M, Rossell, D, Auer, H, Gallardo, M, Blasco, MA, Sancho, E, Clevers, H & Batlle, E 2011, 'Isolation and in vitro expansion of human colonic stem cells', Nature Medicine, vol. 17, no. 10, pp. 1225-1227. https://doi.org/10.1038/nm.2470
Jung P, Sato T, Merlos-Suárez A, Barriga FM, Iglesias M, Rossell D et al. Isolation and in vitro expansion of human colonic stem cells. Nature Medicine. 2011 Oct;17(10):1225-1227. https://doi.org/10.1038/nm.2470
Jung, Peter ; Sato, Toshiro ; Merlos-Suárez, Anna ; Barriga, Francisco M. ; Iglesias, Mar ; Rossell, David ; Auer, Herbert ; Gallardo, Mercedes ; Blasco, Maria A. ; Sancho, Elena ; Clevers, Hans ; Batlle, Eduard. / Isolation and in vitro expansion of human colonic stem cells. In: Nature Medicine. 2011 ; Vol. 17, No. 10. pp. 1225-1227.
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