Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family

Ulla Aapola, Kazunori Shibuya, Hamish S. Scott, Juha Ollila, Mauno Vihinen, Maarit Heino, Ai Shintani, Kazuhiko Kawasaki, Shinsei Minoshima, Kai Krohn, Stylianos E. Antonarakis, Nobuyoshi Shimizu, Jun Kudoh, Pärt Peterson

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

We have isolated the DNMT3L gene that is related to the cytosine-5- methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family. The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)293-298
Number of pages6
JournalGenomics
Volume65
Issue number3
DOIs
Publication statusPublished - 2000 May 1

ASJC Scopus subject areas

  • Genetics

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