Isolation and sequence determination of rat cardiac natriuretic peptide

Yoshikazu Kambayashi, Kazuwa Nakao, Hiroshi Itoh, Kiminori Hosoda, Yoshihiko Saito, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Gotaro Shirakami, Shin ichi Suga, Yoshihiro Ogawa, Michihisa Jougasaki, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Ken Inouye, Hiroo Imura

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.

Original languageEnglish
Pages (from-to)233-240
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume163
Issue number1
DOIs
Publication statusPublished - 1989 Aug 30
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Isolation and sequence determination of rat cardiac natriuretic peptide'. Together they form a unique fingerprint.

  • Cite this

    Kambayashi, Y., Nakao, K., Itoh, H., Hosoda, K., Saito, Y., Yamada, T., Mukoyama, M., Arai, H., Shirakami, G., Suga, S. I., Ogawa, Y., Jougasaki, M., Minamino, N., Kangawa, K., Matsuo, H., Inouye, K., & Imura, H. (1989). Isolation and sequence determination of rat cardiac natriuretic peptide. Biochemical and Biophysical Research Communications, 163(1), 233-240. https://doi.org/10.1016/0006-291X(89)92126-8