Abstract
We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
| Original language | English |
|---|---|
| Pages (from-to) | 233-240 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 163 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1989 Aug 30 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Isolation and sequence determination of rat cardiac natriuretic peptide. / Kambayashi, Yoshikazu; Nakao, Kazuwa; Itoh, Hiroshi; Hosoda, Kiminori; Saito, Yoshihiko; Yamada, Takayuki; Mukoyama, Masashi; Arai, Hiroshi; Shirakami, Gotaro; Suga, Shin ichi; Ogawa, Yoshihiro; Jougasaki, Michihisa; Minamino, Naoto; Kangawa, Kenji; Matsuo, Hisayuki; Inouye, Ken; Imura, Hiroo.
In: Biochemical and Biophysical Research Communications, Vol. 163, No. 1, 30.08.1989, p. 233-240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and sequence determination of rat cardiac natriuretic peptide
AU - Kambayashi, Yoshikazu
AU - Nakao, Kazuwa
AU - Itoh, Hiroshi
AU - Hosoda, Kiminori
AU - Saito, Yoshihiko
AU - Yamada, Takayuki
AU - Mukoyama, Masashi
AU - Arai, Hiroshi
AU - Shirakami, Gotaro
AU - Suga, Shin ichi
AU - Ogawa, Yoshihiro
AU - Jougasaki, Michihisa
AU - Minamino, Naoto
AU - Kangawa, Kenji
AU - Matsuo, Hisayuki
AU - Inouye, Ken
AU - Imura, Hiroo
PY - 1989/8/30
Y1 - 1989/8/30
N2 - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
AB - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
UR - http://www.scopus.com/inward/record.url?scp=0024462264&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024462264&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)92126-8
DO - 10.1016/0006-291X(89)92126-8
M3 - Article
C2 - 2528349
AN - SCOPUS:0024462264
VL - 163
SP - 233
EP - 240
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -