TY - JOUR
T1 - Isolation and sequence determination of rat cardiac natriuretic peptide
AU - Kambayashi, Yoshikazu
AU - Nakao, Kazuwa
AU - Itoh, Hiroshi
AU - Hosoda, Kiminori
AU - Saito, Yoshihiko
AU - Yamada, Takayuki
AU - Mukoyama, Masashi
AU - Arai, Hiroshi
AU - Shirakami, Gotaro
AU - Suga, Shin ichi
AU - Ogawa, Yoshihiro
AU - Jougasaki, Michihisa
AU - Minamino, Naoto
AU - Kangawa, Kenji
AU - Matsuo, Hisayuki
AU - Inouye, Ken
AU - Imura, Hiroo
PY - 1989/8/30
Y1 - 1989/8/30
N2 - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
AB - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
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U2 - 10.1016/0006-291X(89)92126-8
DO - 10.1016/0006-291X(89)92126-8
M3 - Article
C2 - 2528349
AN - SCOPUS:0024462264
VL - 163
SP - 233
EP - 240
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -