Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover

Masaya Imoto, Kazuo Umezawa, Yoshikazu Takahashi, Hiroshi Naganawa, Hikaru Nakamura, Yumi Koizumi, Yumi Sasaki, Masa Hamada, Tsutomu Sawa, Tomio Takeuchi, Yoichi Iitaka

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39 Citations (Scopus)

Abstract

In the course of screening for phosphatidylinosicol turnover inhibitors, we found that a culture broth of a Streptomyces strain strongly inhibited the incorporation of inositol into phosphatidylinositol and its phosphates. The active principle was isolated from the broth through EtOAc extraction, cc, centrifugation partition chromatography, and crystallization. Spectroscopic and crystallographic analysis revealed that it has a novel polyether structure, and we named it inostamycin. Inostamycin inhibited phosphatidylinositol turnover with an IC50 of 0.5 μg/ml in cultured A431 cells.

Original languageEnglish
Pages (from-to)825-829
Number of pages5
JournalJournal of Natural Products
Volume53
Issue number4
DOIs
Publication statusPublished - 1990 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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    Imoto, M., Umezawa, K., Takahashi, Y., Naganawa, H., Nakamura, H., Koizumi, Y., Sasaki, Y., Hamada, M., Sawa, T., Takeuchi, T., & Iitaka, Y. (1990). Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover. Journal of Natural Products, 53(4), 825-829. https://doi.org/10.1021/np50070a008