Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover

Masaya Imoto, Kazuo Umezawa, Yoshikazu Takahashi, Hiroshi Naganawa, Yoichi Iitaka, Hikaru Nakamura, Yumi Koizumi, Yumi Sasaki, Masa Hamada, Tsutomu Sawa, Tomio Takeuchi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In the course of screening for phosphatidylinosicol turnover inhibitors, we found that a culture broth of a Streptomyces strain strongly inhibited the incorporation of inositol into phosphatidylinositol and its phosphates. The active principle was isolated from the broth through EtOAc extraction, cc, centrifugation partition chromatography, and crystallization. Spectroscopic and crystallographic analysis revealed that it has a novel polyether structure, and we named it inostamycin. Inostamycin inhibited phosphatidylinositol turnover with an IC50 of 0.5 μg/ml in cultured A431 cells.

Original languageEnglish
Pages (from-to)825-829
Number of pages5
JournalJournal of Natural Products
Volume53
Issue number4
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

phosphatidylinositols
Phosphatidylinositols
Phosphatidylinositol Phosphates
Centrifugation
Polyethers
Streptomyces
Inositol
crystallization
Crystallization
Chromatography
cultured cells
centrifugation
Inhibitory Concentration 50
inhibitory concentration 50
Cultured Cells
chromatography
Screening
phosphates
screening
inostamycin

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Imoto, M., Umezawa, K., Takahashi, Y., Naganawa, H., Iitaka, Y., Nakamura, H., ... Takeuchi, T. (1990). Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover. Journal of Natural Products, 53(4), 825-829.

Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover. / Imoto, Masaya; Umezawa, Kazuo; Takahashi, Yoshikazu; Naganawa, Hiroshi; Iitaka, Yoichi; Nakamura, Hikaru; Koizumi, Yumi; Sasaki, Yumi; Hamada, Masa; Sawa, Tsutomu; Takeuchi, Tomio.

In: Journal of Natural Products, Vol. 53, No. 4, 1990, p. 825-829.

Research output: Contribution to journalArticle

Imoto, M, Umezawa, K, Takahashi, Y, Naganawa, H, Iitaka, Y, Nakamura, H, Koizumi, Y, Sasaki, Y, Hamada, M, Sawa, T & Takeuchi, T 1990, 'Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover', Journal of Natural Products, vol. 53, no. 4, pp. 825-829.
Imoto, Masaya ; Umezawa, Kazuo ; Takahashi, Yoshikazu ; Naganawa, Hiroshi ; Iitaka, Yoichi ; Nakamura, Hikaru ; Koizumi, Yumi ; Sasaki, Yumi ; Hamada, Masa ; Sawa, Tsutomu ; Takeuchi, Tomio. / Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover. In: Journal of Natural Products. 1990 ; Vol. 53, No. 4. pp. 825-829.
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