Isolation and transplantation of dopaminergic neurons and neural stem cells

Hideyuki Okano, Takahito Yoshizaki, Takuya Shimazaki, Kazunobu Sawamoto

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Although transplantation of mesencephalic tissue is considered a promising therapy for Parkinson's disease (PD), its clinical use is still restricted to a very few cases. A major limiting factor of this therapy is the difficulty of obtaining sufficient quantities of viable embryonic mesencephalic tissue. To overcome this limitation, techniques to produce dopaminergic (DA) neurons in vitro have been developed. However, these cultures are likely to contain a variety of unidentified cells, which must be removed before implantation. Specific cell-surface markers to sort DA neurons or their precursors are not available. We have developed an alternative strategy, by which these cells can be labeled with green fluorescent protein and isolated with fluorescent activated cell sorter. Transplantation of the sorted cells resulted in recovery of a rat model of the PD. This strategy should be useful for developing new therapies for PD.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalParkinsonism and Related Disorders
Volume9
Issue number1
DOIs
Publication statusPublished - 2002 Oct 1

Keywords

  • Dopaminergic neurons
  • Neural stem cells
  • Parkinson's disease

ASJC Scopus subject areas

  • Neurology
  • Geriatrics and Gerontology
  • Clinical Neurology

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