Isolation, drug sensitivity, and some biochemical and genetical properties of macromomycin-resistant mouse lymphoblastoma L5178Y cells

H. Suzuki, Yoshikazu Sugimoto, K. Tanaka, T. Nishimura, N. Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A macromomycin (MCR)-resistant subline of mouse lymphoblastoma L5178Y cells was isolated after successive treatment of tumor-bearing mice with the antibiotic for 7 transplant generations, followed by cloning in culture in MCR-containing soft agar medium. The resistant cell line was about 17 times more resistant to MCR than was the parental cell line and exhibited cross-resistance to neocarzinostatin, mitomycin C and adriamycin in a similar degree to MCR. No significant cross-resistance was observed with aclarubicin, bleomycin and neothramycin. Alkaline phosphodiesterase activity in the plasma membrane of resistant cells was higher than that of parental cells. Uptake and efflux studies with [3H]adriamycin suggested that the resistance is due to decreased uptake and increased efflux of the antibiotic in resistant cells. Hybridization studies with MCR-sensitive and -resistant cells showed that the MCR resistance is a codominant trait in somatic cell hybrids.

Original languageEnglish
Pages (from-to)602-609
Number of pages8
JournalJournal of Antibiotics
Volume37
Issue number5
Publication statusPublished - 1984
Externally publishedYes

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Pharmaceutical Preparations
Doxorubicin
Aclarubicin
Zinostatin
Phosphodiesterase I
Anti-Bacterial Agents
Cell Line
Hybrid Cells
Bleomycin
Mitomycin
Agar
Organism Cloning
Cell Membrane
Transplants
Neoplasms
Therapeutics
neothramycins

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Isolation, drug sensitivity, and some biochemical and genetical properties of macromomycin-resistant mouse lymphoblastoma L5178Y cells. / Suzuki, H.; Sugimoto, Yoshikazu; Tanaka, K.; Nishimura, T.; Tanaka, N.

In: Journal of Antibiotics, Vol. 37, No. 5, 1984, p. 602-609.

Research output: Contribution to journalArticle

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AU - Tanaka, N.

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