Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents

Kwesi Teye, Sanae Numata, Norito Ishii, Rafal P. Krol, Atsunari Tsuchisaka, Takahiro Hamada, Hiroshi Koga, Tadashi Karashima, Chika Ohata, Daisuke Tsuruta, Hideyuki Saya, Marek Haftek, Takashi Hashimoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.

Original languageEnglish
Article numbere0160952
JournalPLoS One
Volume11
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1

Fingerprint

epidermis (animal)
Epidermis
Protein Isoforms
keratinocytes
Keratinocytes
Acrylamide
Cloning
Alternative Splicing
Proteoglycans
Tretinoin
Epidermal Growth Factor
Sepharose
Hydrogen Peroxide
proteoglycans
acrylamides
retinoic acid
alternative splicing
epidermal growth factor
fetal bovine serum
Acetates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Teye, K., Numata, S., Ishii, N., Krol, R. P., Tsuchisaka, A., Hamada, T., ... Hashimoto, T. (2016). Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents. PLoS One, 11(8), [e0160952]. https://doi.org/10.1371/journal.pone.0160952

Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents. / Teye, Kwesi; Numata, Sanae; Ishii, Norito; Krol, Rafal P.; Tsuchisaka, Atsunari; Hamada, Takahiro; Koga, Hiroshi; Karashima, Tadashi; Ohata, Chika; Tsuruta, Daisuke; Saya, Hideyuki; Haftek, Marek; Hashimoto, Takashi.

In: PLoS One, Vol. 11, No. 8, e0160952, 01.08.2016.

Research output: Contribution to journalArticle

Teye, K, Numata, S, Ishii, N, Krol, RP, Tsuchisaka, A, Hamada, T, Koga, H, Karashima, T, Ohata, C, Tsuruta, D, Saya, H, Haftek, M & Hashimoto, T 2016, 'Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents', PLoS One, vol. 11, no. 8, e0160952. https://doi.org/10.1371/journal.pone.0160952
Teye, Kwesi ; Numata, Sanae ; Ishii, Norito ; Krol, Rafal P. ; Tsuchisaka, Atsunari ; Hamada, Takahiro ; Koga, Hiroshi ; Karashima, Tadashi ; Ohata, Chika ; Tsuruta, Daisuke ; Saya, Hideyuki ; Haftek, Marek ; Hashimoto, Takashi. / Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents. In: PLoS One. 2016 ; Vol. 11, No. 8.
@article{58b10d9d455f4bc184d104efbfc29172,
title = "Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents",
abstract = "CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.",
author = "Kwesi Teye and Sanae Numata and Norito Ishii and Krol, {Rafal P.} and Atsunari Tsuchisaka and Takahiro Hamada and Hiroshi Koga and Tadashi Karashima and Chika Ohata and Daisuke Tsuruta and Hideyuki Saya and Marek Haftek and Takashi Hashimoto",
year = "2016",
month = "8",
day = "1",
doi = "10.1371/journal.pone.0160952",
language = "English",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents

AU - Teye, Kwesi

AU - Numata, Sanae

AU - Ishii, Norito

AU - Krol, Rafal P.

AU - Tsuchisaka, Atsunari

AU - Hamada, Takahiro

AU - Koga, Hiroshi

AU - Karashima, Tadashi

AU - Ohata, Chika

AU - Tsuruta, Daisuke

AU - Saya, Hideyuki

AU - Haftek, Marek

AU - Hashimoto, Takashi

PY - 2016/8/1

Y1 - 2016/8/1

N2 - CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.

AB - CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.

UR - http://www.scopus.com/inward/record.url?scp=84983739119&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983739119&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0160952

DO - 10.1371/journal.pone.0160952

M3 - Article

C2 - 27505250

AN - SCOPUS:84983739119

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e0160952

ER -