Isolation of allelopathic substance from Piper sarmentosum Roxb

Pukclai Piyatida, Kiyotake Suenaga, Osamu Ohno, Hisashi Kato-Noguchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Allelopathic activity of Piper sarmentosum Roxb. was determined and a potent growth inhibitory substance was isolated. The aqueous methanol extracts of P. sarmentosum inhibited the growth of 6-test plant species [cress (Lepidium sativum L.), lettuce (Lactuca sativa L.), alfalfa (Medicago sativa L.), timothy (Phleum pretense L.), Italian ryegrass (Lolium multiflorum Lam.) and crabgrass (Digitaria sanguinalis L.)] to various degrees in a dose-dependent manner. The active substance 3-phenylpropionic acid was purified from the aqueous methanol extract of P. sarmentosum and identified by 1H-, 13C-NMR and MS. The threshold of 3-phenylpropionic acid for growth inhibition was 1 μM. The concentrations required for 50% growth inhibition of cress and lettuce were 1.2-9.3 μM and the concentrations required for 50% growth inhibition of timothy and Italian ryegrass were 4.7-51.8 μM. The endogenous concentration of 3-phenylpropionic acid in P. sarmentosum was 121 μmol/kg. Considering the endogenous level and the inhibitory activity, 3-phenylpropionic acid may work as allelopathic substance in P. sarmentosum through growth inhibition of neighbouring plant species.

Original languageEnglish
Pages (from-to)93-102
Number of pages10
JournalAllelopathy Journal
Volume30
Issue number1
Publication statusPublished - 2012 Jul

Fingerprint

Piper
allelochemicals
growth retardation
Lolium multiflorum
acids
lettuce
methanol
Phleum
Digitaria sanguinalis
Lepidium sativum
substance P
allelopathy
extracts
Lactuca sativa
Medicago sativa
alfalfa
water
dosage
testing

Keywords

  • Alfalfa
  • Allelopathic activity
  • Allelopathy
  • Aqueous methanol extract
  • Crabgrass
  • Cress
  • Italian ryegrass
  • Lettuce
  • Piper sarmentosum
  • Timothy

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Piyatida, P., Suenaga, K., Ohno, O., & Kato-Noguchi, H. (2012). Isolation of allelopathic substance from Piper sarmentosum Roxb. Allelopathy Journal, 30(1), 93-102.

Isolation of allelopathic substance from Piper sarmentosum Roxb. / Piyatida, Pukclai; Suenaga, Kiyotake; Ohno, Osamu; Kato-Noguchi, Hisashi.

In: Allelopathy Journal, Vol. 30, No. 1, 07.2012, p. 93-102.

Research output: Contribution to journalArticle

Piyatida, P, Suenaga, K, Ohno, O & Kato-Noguchi, H 2012, 'Isolation of allelopathic substance from Piper sarmentosum Roxb', Allelopathy Journal, vol. 30, no. 1, pp. 93-102.
Piyatida P, Suenaga K, Ohno O, Kato-Noguchi H. Isolation of allelopathic substance from Piper sarmentosum Roxb. Allelopathy Journal. 2012 Jul;30(1):93-102.
Piyatida, Pukclai ; Suenaga, Kiyotake ; Ohno, Osamu ; Kato-Noguchi, Hisashi. / Isolation of allelopathic substance from Piper sarmentosum Roxb. In: Allelopathy Journal. 2012 ; Vol. 30, No. 1. pp. 93-102.
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