Isolation of allelopathic substance from Piper sarmentosum Roxb

Pukclai Piyatida, Kiyotake Suenaga, Osamu Ohno, Hisashi Kato-Noguchi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Allelopathic activity of Piper sarmentosum Roxb. was determined and a potent growth inhibitory substance was isolated. The aqueous methanol extracts of P. sarmentosum inhibited the growth of 6-test plant species [cress (Lepidium sativum L.), lettuce (Lactuca sativa L.), alfalfa (Medicago sativa L.), timothy (Phleum pretense L.), Italian ryegrass (Lolium multiflorum Lam.) and crabgrass (Digitaria sanguinalis L.)] to various degrees in a dose-dependent manner. The active substance 3-phenylpropionic acid was purified from the aqueous methanol extract of P. sarmentosum and identified by 1H-, 13C-NMR and MS. The threshold of 3-phenylpropionic acid for growth inhibition was 1 μM. The concentrations required for 50% growth inhibition of cress and lettuce were 1.2-9.3 μM and the concentrations required for 50% growth inhibition of timothy and Italian ryegrass were 4.7-51.8 μM. The endogenous concentration of 3-phenylpropionic acid in P. sarmentosum was 121 μmol/kg. Considering the endogenous level and the inhibitory activity, 3-phenylpropionic acid may work as allelopathic substance in P. sarmentosum through growth inhibition of neighbouring plant species.

Original languageEnglish
Pages (from-to)93-102
Number of pages10
JournalAllelopathy Journal
Volume30
Issue number1
Publication statusPublished - 2012 Jul 1

Keywords

  • Alfalfa
  • Allelopathic activity
  • Allelopathy
  • Aqueous methanol extract
  • Crabgrass
  • Cress
  • Italian ryegrass
  • Lettuce
  • Piper sarmentosum
  • Timothy

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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