Isolation of cDNA for a novel human protein KNP-I that is homologous to the E. coli SCRP-27A protein from the autoimmune polyglandular disease type I (APECED) region of chromosome 21q22.3

Kentaro Nagamine, Jun Kudo, Shinsei Minoshima, Kazuhiko Kawasaki, Shuichi Asakawa, Fumiaki Ito, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have isolated cDNA clones for a novel human protein KNP-I from fetal brain and bone marrow cDNA libraries. Northern blot analysis indicated that the KNP-I gene is ubiquitously expressed in various human tissues. Significant homology of the KNP-I protein with Escherichia coli anti-sigma cross-reacting protein (SCRP-27A) (44% identity) and zebrafish (Brachydanio rerio) es1 protein (49% identity) suggested that the KNP-I protein may be involved in a basic cellular function. Genomic sequencing revealed that the KNP-I gene consists of seven exons spanning 12 kb. Exon 5 was involved in alternative splicing. The KNP-I gene was mapped between D21S1460 and D21S25 on human chromosome 21q22.3, 26 kb distal to a Not I site of D21S1460. Thus, this novel KNP-I gene could be a candidate gene for autoimmune polyglandular disease type I (APECED) and other disorders mapped to this region.

Original languageEnglish
Pages (from-to)608-616
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume225
Issue number2
DOIs
Publication statusPublished - 1996 Aug 14

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Autoimmune Polyendocrinopathies
Chromosomes
Autoimmune Diseases
Complementary DNA
Genes
Proteins
Exons
Zebrafish Proteins
Escherichia coli Proteins
Alternative Splicing
Human Chromosomes
Zebrafish
Gene Library
Northern Blotting
Escherichia coli
Brain
Bone
Clone Cells
Bone Marrow
E coli elbB protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Isolation of cDNA for a novel human protein KNP-I that is homologous to the E. coli SCRP-27A protein from the autoimmune polyglandular disease type I (APECED) region of chromosome 21q22.3. / Nagamine, Kentaro; Kudo, Jun; Minoshima, Shinsei; Kawasaki, Kazuhiko; Asakawa, Shuichi; Ito, Fumiaki; Shimizu, Nobuyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 225, No. 2, 14.08.1996, p. 608-616.

Research output: Contribution to journalArticle

Nagamine, Kentaro ; Kudo, Jun ; Minoshima, Shinsei ; Kawasaki, Kazuhiko ; Asakawa, Shuichi ; Ito, Fumiaki ; Shimizu, Nobuyoshi. / Isolation of cDNA for a novel human protein KNP-I that is homologous to the E. coli SCRP-27A protein from the autoimmune polyglandular disease type I (APECED) region of chromosome 21q22.3. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 225, No. 2. pp. 608-616.
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AU - Minoshima, Shinsei

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AU - Shimizu, Nobuyoshi

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