Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

We report the presence of neural crest-derived corneal precursors (COPs) that initiate spheres by clonal expansion from a single cell. COPs expressed the stem cell markers nestin, Notch1, Musashi-1, and ABCG2 and showed the side population cell phenotype. COPs were multipotent with the ability to differentiate into adipocytes, chondrocytes, as well as neural cells, as shown by the expression of β-III-tubulin, glial fibrillary acidic protein, and neurofilament-M. COP spheres prepared from E/nestin-enhanced green fluorescent protein (EGFP) mice showed induction of EGFP expression that was not originally observed in the cornea, indicating activation of the neural-specific nestin second intronic enhancer in culture. COPs were Sca-1+, CD34 +, CD45-, and c-kit-. Numerous GFP+ cells were observed in the corneas of mice transplanted with whole bone marrow of transgenic mice ubiquitously expressing GFP; however, no GFP+ COP spheres were initiated from these mice. On the other hand, COP spheres from transgenic mice encoding P0-Cre/Floxed-EGFP as well as Wnt1-Cre/Floxed-EGFP were GFP+, indicating the neural crest origin of COPs, which was confirmed by the expression of the embryonic neural crest markers Twist, Snail, Slug, and Sox9. Taken together, these data indicate the existence of neural crest-derived, multipotent stem cells in the adult cornea.

Original languageEnglish
Pages (from-to)2714-2722
Number of pages9
JournalStem Cells
Volume24
Issue number12
DOIs
Publication statusPublished - 2006 Dec

Fingerprint

Adult Stem Cells
Neural Crest
Nestin
Cornea
Transgenic Mice
Side-Population Cells
Multipotent Stem Cells
Gastropoda
Intermediate Filaments
Glial Fibrillary Acidic Protein
Tubulin
Chondrocytes
Adipocytes
Stem Cells
Bone Marrow
Phenotype
enhanced green fluorescent protein

Keywords

  • Bone marrow cells
  • Corneal stroma
  • Keratocyte
  • Neural crest
  • Stem cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea. / Yoshida, Satoru; Shimmura, Shigeto; Nagoshi, Narihito; Fukuda, Keiichi; Matsuzaki, Yumi; Okano, Hideyuki; Tsubota, Kazuo.

In: Stem Cells, Vol. 24, No. 12, 12.2006, p. 2714-2722.

Research output: Contribution to journalArticle

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