Isolation of potential probiotic Lactobacillus rhamnosus strains from traditional fermented mare milk produced in Sumbawa Island of Indonesia

Tala Shi, Keita Nishiyama, Koichi Nakamata, Ni Putu Desy Aryantini, Dai Mikumo, Yuji Oda, Yuji Yamamoto, Takao Mukai, I. Nengah Sujaya, Tadasu Urashima, Kenji Fukuda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To explore potential probiotics in the traditional foods of Indonesia, fermented mare milk produced in Sumbawa Island was investigated in this study. Gram stain, catalase activity, gas production, cell morphology, carbohydrate utilization pattern, and 16S rDNA sequencing were performed to identify isolated lactic acid bacteria. To assess their probiotic ability, tolerance of low pH, bile salts, artificial gastrointestinal fluids, and adhesion properties to extracellular matrices, were examined. In total 27 strains, 25 Lactobacillus rhamnosus and two Lactobacillus fermentum, were obtained. Among the isolated lactobacilli, three Lb. rhamnosus strains, FSMM15, FSMM22, and FSMM26, were selected as candidates for probiotics, using Lb. rhamnosus GG as index. In vitro binding assay of the three strains against several extracellular matrix proteins revealed that FSMM15 and FSMM26 gave greater binding ratios of mucin/bovine serum albumin (BSA) and significantly higher adhesive abilities to fibronectin than Lb. rhamnosus GG. FSMM22 showed significantly higher adhesion to laminin than Lb. rhamnosus GG.

Original languageEnglish
Pages (from-to)1897-1903
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number10
DOIs
Publication statusPublished - 2012 Nov 12
Externally publishedYes

Fingerprint

Fermented milk
Lactobacillus rhamnosus
Indonesia
Probiotics
Islands
Milk
Adhesion
Lactobacillus fermentum
Extracellular Matrix Proteins
Lactobacillus
Laminin
Mucins
Bovine Serum Albumin
Ribosomal DNA
Bile Acids and Salts
Fibronectins
Adhesives
Catalase
Extracellular Matrix
Lactic Acid

Keywords

  • Lactobacillus rhamnosus
  • Laminin adhesive ability
  • Probiotic properties
  • Sumbawa mare
  • Traditional fermented milk

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Isolation of potential probiotic Lactobacillus rhamnosus strains from traditional fermented mare milk produced in Sumbawa Island of Indonesia. / Shi, Tala; Nishiyama, Keita; Nakamata, Koichi; Aryantini, Ni Putu Desy; Mikumo, Dai; Oda, Yuji; Yamamoto, Yuji; Mukai, Takao; Sujaya, I. Nengah; Urashima, Tadasu; Fukuda, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 10, 12.11.2012, p. 1897-1903.

Research output: Contribution to journalArticle

Shi, T, Nishiyama, K, Nakamata, K, Aryantini, NPD, Mikumo, D, Oda, Y, Yamamoto, Y, Mukai, T, Sujaya, IN, Urashima, T & Fukuda, K 2012, 'Isolation of potential probiotic Lactobacillus rhamnosus strains from traditional fermented mare milk produced in Sumbawa Island of Indonesia', Bioscience, Biotechnology and Biochemistry, vol. 76, no. 10, pp. 1897-1903. https://doi.org/10.1271/bbb.120385
Shi, Tala ; Nishiyama, Keita ; Nakamata, Koichi ; Aryantini, Ni Putu Desy ; Mikumo, Dai ; Oda, Yuji ; Yamamoto, Yuji ; Mukai, Takao ; Sujaya, I. Nengah ; Urashima, Tadasu ; Fukuda, Kenji. / Isolation of potential probiotic Lactobacillus rhamnosus strains from traditional fermented mare milk produced in Sumbawa Island of Indonesia. In: Bioscience, Biotechnology and Biochemistry. 2012 ; Vol. 76, No. 10. pp. 1897-1903.
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