Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168

Mitsuhiro Itaya, Akira Omori, Shigenori Kanaya, Robert J. Crouch, Teruo Tanaka, Kanae Kondo

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Two genes encoding functional RNase H (EC 3.1.26.4) were isolated from a gram-positive bacterium, Bacillus subtilis 168. Two DNA clones exhibiting RNase H activities both in vivo and in vitro were obtained from a B. subtilis DNA library. One (28.2 kDa) revealed high similarity to Escherichia coli RNase HII, encoded by the rnhB gene. The other (33.9 kDa) was designated rnhC and encodes B. subtilis RNase HIII. The B. subtilis genome has an rnhA homologue, the product of which has not yet shown RNase H activity. Analyses of all three B. subtilis genes revealed that rnhB and rnhC cannot be simultaneously inactivated. This observation indicated that in B. subtilis both the rnhB and rnhC products are involved in certain essential cellular processes that are different from those suggested by E. coli rnh mutation studies. Sequence conservation between the rnhB and rnhC genes implies that both originated from a single ancestral RNase H gene. The roles of bacterial RNase H may be indicated by the single rnhC homologue in the small genome of Mycoplasma species.

Original languageEnglish
Pages (from-to)2118-2123
Number of pages6
JournalJournal of Bacteriology
Volume181
Issue number7
Publication statusPublished - 1999 Apr
Externally publishedYes

Fingerprint

Ribonuclease H
Essential Genes
Bacillus subtilis
Growth
Genes
Genome
Escherichia coli
Mycoplasma
Gram-Positive Bacteria
Gene Library
Clone Cells
Mutation
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Itaya, M., Omori, A., Kanaya, S., Crouch, R. J., Tanaka, T., & Kondo, K. (1999). Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. Journal of Bacteriology, 181(7), 2118-2123.

Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. / Itaya, Mitsuhiro; Omori, Akira; Kanaya, Shigenori; Crouch, Robert J.; Tanaka, Teruo; Kondo, Kanae.

In: Journal of Bacteriology, Vol. 181, No. 7, 04.1999, p. 2118-2123.

Research output: Contribution to journalArticle

Itaya, M, Omori, A, Kanaya, S, Crouch, RJ, Tanaka, T & Kondo, K 1999, 'Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168', Journal of Bacteriology, vol. 181, no. 7, pp. 2118-2123.
Itaya M, Omori A, Kanaya S, Crouch RJ, Tanaka T, Kondo K. Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. Journal of Bacteriology. 1999 Apr;181(7):2118-2123.
Itaya, Mitsuhiro ; Omori, Akira ; Kanaya, Shigenori ; Crouch, Robert J. ; Tanaka, Teruo ; Kondo, Kanae. / Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 7. pp. 2118-2123.
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