Japanese external quality assessment program to standardize HIV-1 drug-resistance testing (JEQS2010 program) using in vitro transcribed RNA as reference material

Shigeru Yoshida, Junko Hattori, Masakazu Matsuda, Kiyomi Okada, Yukumasa Kazuyama, Osamu Hashimoto, Shiro Ibe, Shin Ichi Fujisawa, Hitoshi Chiba, Masashi Tatsumi, Shingo Kato, Wataru Sugiura

Research output: Contribution to journalArticle

Abstract

To design appropriate antiretroviral therapy regimens and avoid the emergence of human immunodeficiency virus (HIV)-1 variants with reduced susceptibility to antiretroviral drugs, genotypic drug-resistance testing (HIV genotyping) is strongly recommended. To monitor the quality of HIV genotyping in Japan, we performed an external quality assessment (EQA), named the Japanese external quality assessment program, to standardize HIV genotyping (JEQS). To accurately evaluate the quality of HIV genotyping, we employed as reference material (RM) a well-characterized sample, in vitro transcribed RNA (trRNA) that includes the HIV gag-pol sequence, and created a JEQS2010 panel consisting of three single variant and three mixed trRNA samples. All 11 participating laboratories showed high concordance rates (>96%) for the single variant samples. Eight laboratories also showed good rates of detecting minor variants, but three laboratories failed to detect the variants comprising one-half of the sample. These three laboratories used a common primer that had four internal mismatches to the minor trRNA clone. This program showed the usefulness of trRNA as RM, the high quality of HIV genotyping, and extensive interlaboratory variation in the ability to detect minor variants. These results suggest that improving the quality of HIV genotyping in Japan requires regularly implementing the EQA program and improving the HIV genotyping protocol in each laboratory.

Original languageEnglish
Pages (from-to)318-325
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume31
Issue number3
DOIs
Publication statusPublished - 2015 Mar 1

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Drug Resistance
HIV-1
HIV
RNA
Japan
In Vitro Techniques
Clone Cells
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Japanese external quality assessment program to standardize HIV-1 drug-resistance testing (JEQS2010 program) using in vitro transcribed RNA as reference material. / Yoshida, Shigeru; Hattori, Junko; Matsuda, Masakazu; Okada, Kiyomi; Kazuyama, Yukumasa; Hashimoto, Osamu; Ibe, Shiro; Fujisawa, Shin Ichi; Chiba, Hitoshi; Tatsumi, Masashi; Kato, Shingo; Sugiura, Wataru.

In: AIDS Research and Human Retroviruses, Vol. 31, No. 3, 01.03.2015, p. 318-325.

Research output: Contribution to journalArticle

Yoshida, S, Hattori, J, Matsuda, M, Okada, K, Kazuyama, Y, Hashimoto, O, Ibe, S, Fujisawa, SI, Chiba, H, Tatsumi, M, Kato, S & Sugiura, W 2015, 'Japanese external quality assessment program to standardize HIV-1 drug-resistance testing (JEQS2010 program) using in vitro transcribed RNA as reference material', AIDS Research and Human Retroviruses, vol. 31, no. 3, pp. 318-325. https://doi.org/10.1089/aid.2014.0059
Yoshida, Shigeru ; Hattori, Junko ; Matsuda, Masakazu ; Okada, Kiyomi ; Kazuyama, Yukumasa ; Hashimoto, Osamu ; Ibe, Shiro ; Fujisawa, Shin Ichi ; Chiba, Hitoshi ; Tatsumi, Masashi ; Kato, Shingo ; Sugiura, Wataru. / Japanese external quality assessment program to standardize HIV-1 drug-resistance testing (JEQS2010 program) using in vitro transcribed RNA as reference material. In: AIDS Research and Human Retroviruses. 2015 ; Vol. 31, No. 3. pp. 318-325.
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