TY - JOUR
T1 - Japanese herbal medicine, Saiko-keishi-to, prevents gut ischemia/reperfusion-induced liver injury in rats via nitric oxide
AU - Horie, Yoshinori
AU - Kajihara, Mikio
AU - Mori, Shuka
AU - Yamagishi, Yoshiyuki
AU - Kimura, Hiroyuki
AU - Tamai, Hironao
AU - Kato, Shinzo
AU - Ishii, Hiromasa
PY - 2004/8/1
Y1 - 2004/8/1
N2 - Aim: To determine whether Saiko-keishi-to (TJ-10), a Japanese herbal medicine, could protect liver injury induced by gut ischemia/reperfusion (I/R), and to investigate the role of NO. Methods: Male Wistar rats were exposed to 30-min gut ischemia followed by 60 min of reperfusion. Intravital microscopy was used to monitor leukocyte recruitment. Plasma tumor necrosis factor (TNF) levels and alanine aminotransferase (ALT) activities were measured. TJ-10 1 g/(kg·d) was intragastrically administered to rats for 7 d. A NO synthase inhibitor was administered. Results: In control rats, gut I/R elicited increases in the number of stationary leukocytes, and plasma TNF levels and ALT activities were mitigated by pretreatment with TJ-10. Pretreatment with the NO synthase inhibitor diminished the protective effects of TJ-10 on leukostasis in the liver, and the increase of plasma TNF levels and ALT activities. Pretreatment with TJ-10 increased plasma nitrite/nitrate levels. Conclusion: TJ-10 attenuates the gut I/R-induced hepatic microvascular dysfunction and sequential hepatocellular injury via enhancement of NO production.
AB - Aim: To determine whether Saiko-keishi-to (TJ-10), a Japanese herbal medicine, could protect liver injury induced by gut ischemia/reperfusion (I/R), and to investigate the role of NO. Methods: Male Wistar rats were exposed to 30-min gut ischemia followed by 60 min of reperfusion. Intravital microscopy was used to monitor leukocyte recruitment. Plasma tumor necrosis factor (TNF) levels and alanine aminotransferase (ALT) activities were measured. TJ-10 1 g/(kg·d) was intragastrically administered to rats for 7 d. A NO synthase inhibitor was administered. Results: In control rats, gut I/R elicited increases in the number of stationary leukocytes, and plasma TNF levels and ALT activities were mitigated by pretreatment with TJ-10. Pretreatment with the NO synthase inhibitor diminished the protective effects of TJ-10 on leukostasis in the liver, and the increase of plasma TNF levels and ALT activities. Pretreatment with TJ-10 increased plasma nitrite/nitrate levels. Conclusion: TJ-10 attenuates the gut I/R-induced hepatic microvascular dysfunction and sequential hepatocellular injury via enhancement of NO production.
UR - http://www.scopus.com/inward/record.url?scp=4344626871&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4344626871&partnerID=8YFLogxK
U2 - 10.3748/wjg.v10.i15.2241
DO - 10.3748/wjg.v10.i15.2241
M3 - Article
C2 - 15259073
AN - SCOPUS:4344626871
SN - 1007-9327
VL - 10
SP - 2241
EP - 2244
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 15
ER -