K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes

Yutaka Hattori, Hiroki Odagiri, Hiroshi Nakatani, Kiyoshi Miyagawa, Kenichiro Naito, Hiromi Sakamoto, Osamu Katoh, Teruhiko Yoshida, Takashi Sugimura, Masaaki Terada

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam (KATO-III cell-derived stomach cancer amplified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.

Original languageEnglish
Pages (from-to)5983-5987
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number15
Publication statusPublished - 1990
Externally publishedYes

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Fibroblast Growth Factor Receptors
Stomach Neoplasms
Genes
Germ Cell and Embryonal Neoplasms
Neoplasm Genes
DNA
Small Cell Lung Carcinoma
Receptor Protein-Tyrosine Kinases
Fibroblast Growth Factor 2
Short Stature, Auditory Canal Atresia, Mandibular Hypoplasia, Skeletal Abnormalities
Walking
Sequence Analysis
Chickens
Exons
Complementary DNA
Gels
Amino Acids
Cell Line
Messenger RNA

Keywords

  • Amplification
  • Fibroblast growth factor
  • Gastric cancer
  • In-gel DNA renaturation method

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes. / Hattori, Yutaka; Odagiri, Hiroki; Nakatani, Hiroshi; Miyagawa, Kiyoshi; Naito, Kenichiro; Sakamoto, Hiromi; Katoh, Osamu; Yoshida, Teruhiko; Sugimura, Takashi; Terada, Masaaki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 15, 1990, p. 5983-5987.

Research output: Contribution to journalArticle

Hattori, Y, Odagiri, H, Nakatani, H, Miyagawa, K, Naito, K, Sakamoto, H, Katoh, O, Yoshida, T, Sugimura, T & Terada, M 1990, 'K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes', Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 15, pp. 5983-5987.
Hattori, Yutaka ; Odagiri, Hiroki ; Nakatani, Hiroshi ; Miyagawa, Kiyoshi ; Naito, Kenichiro ; Sakamoto, Hiromi ; Katoh, Osamu ; Yoshida, Teruhiko ; Sugimura, Takashi ; Terada, Masaaki. / K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 15. pp. 5983-5987.
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AU - Naito, Kenichiro

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AU - Katoh, Osamu

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AU - Sugimura, Takashi

AU - Terada, Masaaki

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