K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes

Yutaka Hattori, Hiroki Odagiri, Hiroshi Nakatani, Kiyoshi Miyagawa, Kenichiro Naito, Hiromi Sakamoto, Osamu Katoh, Teruhiko Yoshida, Takashi Sugimura, Masaaki Terada

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam (KATO-III cell-derived stomach cancer amplified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.

Original languageEnglish
Pages (from-to)5983-5987
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number15
DOIs
Publication statusPublished - 1990 Jan 1

Keywords

  • Amplification
  • Fibroblast growth factor
  • Gastric cancer
  • In-gel DNA renaturation method

ASJC Scopus subject areas

  • General

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