Karyotype evolution and multilineage involvement of Philadelphia chromosome-positive clones in blastic transformation of two patients with chronic myelocytic leukemia

Y. Sato, K. Kitano, S. Tsunoda, M. Yoshida, E. Kajii, T. Suda, S. Sakamoto, K. Motoyoshi, M. Saito, Y. Miura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Simultaneous analysis of the cell morphology and karyotypes on single colonies was carried out in two patients with Philadelphia chromosome (Ph1)-positive chronic myelocytic leukemia in blastic transformation in order to clarify the origin of leukemic cells involved. Patient no. 1 was in a typical myeloblastic transformation and patient no. 2 in 'basophilic transformation'. Both patients exhibited karyotype evolution in blastic phase (BP), so that we could differentiate BP clones with additional chromosomal abnormalities from chronic phase (CP) clones with only Ph1 among single colonies. The number of single colonies yielding two or more analyzable metaphases was 18 in patient no. 1, and 19 in patient no. 2. Among these colonies, only three in patient no. 1 and none in patient no. 2 were from CP clones and 15 in patient no. 1 and 19 in patient no. 2 were from BP clones. Morphological examination revealed that not only blasts but also mature neutrophils, eosinophils, basophils, macrophages, and erythroblasts were derived from BP clones. These results suggested that (1) BP clones developed at the pluripotent stem cell level, (2) additional chromosomal abnormalities were not restricted to occur in a specific cell line representative in BP; and (3) BP clones, if not all, may retain capacity for maturation and differentiation.

Original languageEnglish
Pages (from-to)1561-1567
Number of pages7
JournalBlood
Volume71
Issue number6
Publication statusPublished - 1988

Fingerprint

Philadelphia Chromosome
Macrophages
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Chromosomes
Stem cells
Karyotype
Clone Cells
Cells
Chromosome Aberrations
Erythroblasts
Pluripotent Stem Cells
Basophils
Metaphase
Eosinophils
Neutrophils
Cell Line

ASJC Scopus subject areas

  • Hematology

Cite this

Karyotype evolution and multilineage involvement of Philadelphia chromosome-positive clones in blastic transformation of two patients with chronic myelocytic leukemia. / Sato, Y.; Kitano, K.; Tsunoda, S.; Yoshida, M.; Kajii, E.; Suda, T.; Sakamoto, S.; Motoyoshi, K.; Saito, M.; Miura, Y.

In: Blood, Vol. 71, No. 6, 1988, p. 1561-1567.

Research output: Contribution to journalArticle

Sato, Y, Kitano, K, Tsunoda, S, Yoshida, M, Kajii, E, Suda, T, Sakamoto, S, Motoyoshi, K, Saito, M & Miura, Y 1988, 'Karyotype evolution and multilineage involvement of Philadelphia chromosome-positive clones in blastic transformation of two patients with chronic myelocytic leukemia', Blood, vol. 71, no. 6, pp. 1561-1567.
Sato, Y. ; Kitano, K. ; Tsunoda, S. ; Yoshida, M. ; Kajii, E. ; Suda, T. ; Sakamoto, S. ; Motoyoshi, K. ; Saito, M. ; Miura, Y. / Karyotype evolution and multilineage involvement of Philadelphia chromosome-positive clones in blastic transformation of two patients with chronic myelocytic leukemia. In: Blood. 1988 ; Vol. 71, No. 6. pp. 1561-1567.
@article{a508c097ab0c42ef9b9fc9aed253a91b,
title = "Karyotype evolution and multilineage involvement of Philadelphia chromosome-positive clones in blastic transformation of two patients with chronic myelocytic leukemia",
abstract = "Simultaneous analysis of the cell morphology and karyotypes on single colonies was carried out in two patients with Philadelphia chromosome (Ph1)-positive chronic myelocytic leukemia in blastic transformation in order to clarify the origin of leukemic cells involved. Patient no. 1 was in a typical myeloblastic transformation and patient no. 2 in 'basophilic transformation'. Both patients exhibited karyotype evolution in blastic phase (BP), so that we could differentiate BP clones with additional chromosomal abnormalities from chronic phase (CP) clones with only Ph1 among single colonies. The number of single colonies yielding two or more analyzable metaphases was 18 in patient no. 1, and 19 in patient no. 2. Among these colonies, only three in patient no. 1 and none in patient no. 2 were from CP clones and 15 in patient no. 1 and 19 in patient no. 2 were from BP clones. Morphological examination revealed that not only blasts but also mature neutrophils, eosinophils, basophils, macrophages, and erythroblasts were derived from BP clones. These results suggested that (1) BP clones developed at the pluripotent stem cell level, (2) additional chromosomal abnormalities were not restricted to occur in a specific cell line representative in BP; and (3) BP clones, if not all, may retain capacity for maturation and differentiation.",
author = "Y. Sato and K. Kitano and S. Tsunoda and M. Yoshida and E. Kajii and T. Suda and S. Sakamoto and K. Motoyoshi and M. Saito and Y. Miura",
year = "1988",
language = "English",
volume = "71",
pages = "1561--1567",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "6",

}

TY - JOUR

T1 - Karyotype evolution and multilineage involvement of Philadelphia chromosome-positive clones in blastic transformation of two patients with chronic myelocytic leukemia

AU - Sato, Y.

AU - Kitano, K.

AU - Tsunoda, S.

AU - Yoshida, M.

AU - Kajii, E.

AU - Suda, T.

AU - Sakamoto, S.

AU - Motoyoshi, K.

AU - Saito, M.

AU - Miura, Y.

PY - 1988

Y1 - 1988

N2 - Simultaneous analysis of the cell morphology and karyotypes on single colonies was carried out in two patients with Philadelphia chromosome (Ph1)-positive chronic myelocytic leukemia in blastic transformation in order to clarify the origin of leukemic cells involved. Patient no. 1 was in a typical myeloblastic transformation and patient no. 2 in 'basophilic transformation'. Both patients exhibited karyotype evolution in blastic phase (BP), so that we could differentiate BP clones with additional chromosomal abnormalities from chronic phase (CP) clones with only Ph1 among single colonies. The number of single colonies yielding two or more analyzable metaphases was 18 in patient no. 1, and 19 in patient no. 2. Among these colonies, only three in patient no. 1 and none in patient no. 2 were from CP clones and 15 in patient no. 1 and 19 in patient no. 2 were from BP clones. Morphological examination revealed that not only blasts but also mature neutrophils, eosinophils, basophils, macrophages, and erythroblasts were derived from BP clones. These results suggested that (1) BP clones developed at the pluripotent stem cell level, (2) additional chromosomal abnormalities were not restricted to occur in a specific cell line representative in BP; and (3) BP clones, if not all, may retain capacity for maturation and differentiation.

AB - Simultaneous analysis of the cell morphology and karyotypes on single colonies was carried out in two patients with Philadelphia chromosome (Ph1)-positive chronic myelocytic leukemia in blastic transformation in order to clarify the origin of leukemic cells involved. Patient no. 1 was in a typical myeloblastic transformation and patient no. 2 in 'basophilic transformation'. Both patients exhibited karyotype evolution in blastic phase (BP), so that we could differentiate BP clones with additional chromosomal abnormalities from chronic phase (CP) clones with only Ph1 among single colonies. The number of single colonies yielding two or more analyzable metaphases was 18 in patient no. 1, and 19 in patient no. 2. Among these colonies, only three in patient no. 1 and none in patient no. 2 were from CP clones and 15 in patient no. 1 and 19 in patient no. 2 were from BP clones. Morphological examination revealed that not only blasts but also mature neutrophils, eosinophils, basophils, macrophages, and erythroblasts were derived from BP clones. These results suggested that (1) BP clones developed at the pluripotent stem cell level, (2) additional chromosomal abnormalities were not restricted to occur in a specific cell line representative in BP; and (3) BP clones, if not all, may retain capacity for maturation and differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0023929541&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023929541&partnerID=8YFLogxK

M3 - Article

C2 - 3163504

AN - SCOPUS:0023929541

VL - 71

SP - 1561

EP - 1567

JO - Blood

JF - Blood

SN - 0006-4971

IS - 6

ER -