Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells

Ryuji Morizane, Toshiaki Monkawa, Shizuka Fujii, Shintaro Yamaguchi, Koichiro Honma, Yumi Matsuzaki, Hideyuki Okano, Hiroshi Itoh

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Embryonic stem cells and induced pluripotent stem cells have the ability to differentiate into various organs and tissues, and are regarded as new tools for the elucidation of disease mechanisms as well as sources for regenerative therapies. However, a method of inducing organ-specific cells from pluripotent stem cells is urgently needed. Although many scientists have been developing methods to induce various organ-specific cells from pluripotent stem cells, renal lineage cells have yet to be induced in vitro because of the complexity of kidney structures and the diversity of kidney-component cells. Here, we describe a method of inducing renal tubular cells from mouse embryonic stem cells via the cell purification of kidney specific protein (KSP)-positive cells using an anti-KSP antibody. The global gene expression profiles of KSP-positive cells derived from ES cells exhibited characteristics similar to those of cells in the developing kidney, and KSP-positive cells had the capacity to form tubular structures resembling renal tubular cells when grown in a 3D culture in Matrigel. Moreover, our results indicated that KSP-positive cells acquired the characteristics of each segment of renal tubular cells through tubular formation when stimulated with Wnt4. This method is an important step toward kidney disease research using pluripotent stem cells, and the development of kidney regeneration therapies.

Original languageEnglish
Article numbere64843
JournalPLoS One
Volume8
Issue number6
DOIs
Publication statusPublished - 2013 Jun 3

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embryonic stem cells
kidney cells
Embryonic Stem Cells
Stem cells
Cells
kidneys
Kidney
Proteins
proteins
cells
Pluripotent Stem Cells
stem cells
Gene expression
Purification
In Vitro Techniques
Tissue
therapeutics
Induced Pluripotent Stem Cells
Antibodies
kidney diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells. / Morizane, Ryuji; Monkawa, Toshiaki; Fujii, Shizuka; Yamaguchi, Shintaro; Honma, Koichiro; Matsuzaki, Yumi; Okano, Hideyuki; Itoh, Hiroshi.

In: PLoS One, Vol. 8, No. 6, e64843, 03.06.2013.

Research output: Contribution to journalArticle

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