Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations

T. Kawata, M. Durante, K. George, Y. Furusawa, E. Gotoh, N. Takai, H. Wu, F. A. Cucinotta

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The purpose of this study is to determine the kinetics of chromatid break rejoining following exposure to radiations of different quality. Exponentially growing human fibroblast cells AG1522 were irradiated with γ-rays, energetic carbon (290 MeV/u), silicon (490 MeV/u) and iron (200 MeV/u, 600 MeV/u). Chromosomes were prematurely condensed using calyculin A. Prematurely condensed chromosomes were collected after several post-irradiation incubation times, ranging from 5 to 600 minutes, and the number of chromatid breaks and exchanges in G2 cells were scored. The relative biological effectiveness (RBE) for initial chromatid breaks per unit dose showed LET dependency having a peak at 55 keV/μm silicon (2.4) or 80 keV/μm carbon particles (2.4) and then decreased with increasing LET. The kinetics of chromatid break rejoining following low- or high-LET irradiation consisted of two exponential components. Chromatid breaks decreased rapidly after exposure, and then continued to decrease at a slower rate. The rejoining kinetics was similar for exposure to each type of radiation, although the rate of unrejoined breaks was higher for highLET radiation. Chromatid exchanges were also formed quickly.

Original languageEnglish
Pages (from-to)226-228
Number of pages3
JournalPhysica Medica
Volume17
Issue numberSUPPL. 1
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Linear Energy Transfer
Chromatids
fibroblasts
Fibroblasts
chromosomes
Radiation
kinetics
radiation
irradiation
carbon
silicon
Silicon
rays
Carbon
Chromosomes
Relative Biological Effectiveness
iron
dosage
cells
Iron

Keywords

  • Chromatid breaks and exchanges
  • High-LET radiations
  • Premature chromosome condensation

ASJC Scopus subject areas

  • Biophysics
  • Physics and Astronomy(all)

Cite this

Kawata, T., Durante, M., George, K., Furusawa, Y., Gotoh, E., Takai, N., ... Cucinotta, F. A. (2001). Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations. Physica Medica, 17(SUPPL. 1), 226-228.

Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations. / Kawata, T.; Durante, M.; George, K.; Furusawa, Y.; Gotoh, E.; Takai, N.; Wu, H.; Cucinotta, F. A.

In: Physica Medica, Vol. 17, No. SUPPL. 1, 2001, p. 226-228.

Research output: Contribution to journalArticle

Kawata, T, Durante, M, George, K, Furusawa, Y, Gotoh, E, Takai, N, Wu, H & Cucinotta, FA 2001, 'Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations', Physica Medica, vol. 17, no. SUPPL. 1, pp. 226-228.
Kawata T, Durante M, George K, Furusawa Y, Gotoh E, Takai N et al. Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations. Physica Medica. 2001;17(SUPPL. 1):226-228.
Kawata, T. ; Durante, M. ; George, K. ; Furusawa, Y. ; Gotoh, E. ; Takai, N. ; Wu, H. ; Cucinotta, F. A. / Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations. In: Physica Medica. 2001 ; Vol. 17, No. SUPPL. 1. pp. 226-228.
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