Kupffer cell-independent acute hepatocellular oxidative stress and decreased bile formation in post-cold-ischemic rat liver

Yusuke Kumamoto, Makoto Suematsu, Motohide Shimazu, Yutaro Kato, Tsuyoshi Sano, Nobuya Makino, Ken Ichiro Hirano, Makoto Naito, G. O. Wakabayashi, Yuzuru Ishimura, Masaki Kitajima

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Abstract

The purpose of this study was to examine distribution and time history of oxidative stress during the hyperacute period of reperfusion in the liver grafts undergoing cold ischemia and to investigate roles of Kupffer cells as a potential oxidant source. Rat livers were harvested at 4 °C in University of Wisconsin solution and followed by reperfusion with Krebs-Henseleit buffer under monitoring bile excretion. To investigate oxidative changes, laser- confocal microfluorography was performed in reperfused livers preloaded with dichlorodihydrofluorescein diacetate succinimidyl ester, a fluorescence precursor sensing intracellular hydroperoxide generation. Livers undergoing the 16-hour cold storage displayed an impaired recovery of bile acid- dependent bile output concurrent with a marked increase in hydroperoxide generation in hepatocytes, which occurred as early as 5 minutes after the onset of reperfusion, whereas the status of lobular perfusion was well maintained. Pretreatment with liposome-encapsulated dichloromethylene diphosphonate, a Kupffer cell-depleting reagent, did neither alter the reperfusion-induced periportal oxidative changes nor improve the recovery of bile output in the graft. On the other hand, EPCK, a hepatotropic antioxidant composed of Vitamin E phosphate ester bound to vitamin C, not only diminished the oxidative changes but also improved the reduction of bile acid-dependent bile output. Furthermore, the reagent was capable of inhibiting H2O2- induced oxidative stress in cultured hepatocytes. These results suggest that hepatocytes constitute a major site of the oxidative insult triggered through Kupffer cell-independent mechanisms and serve as an important cellular component to be protected by antioxidant therapeutics.

Original languageEnglish
Pages (from-to)1454-1463
Number of pages10
JournalHepatology
Volume30
Issue number6
Publication statusPublished - 1999

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Kupffer Cells
Bile
Reperfusion
Oxidative Stress
Hepatocytes
Liver
Bile Acids and Salts
Hydrogen Peroxide
Esters
Antioxidants
Transplants
Cold Ischemia
Vitamin E
Oxidants
Ascorbic Acid
Lasers
Perfusion
Fluorescence
Phosphates
Therapeutics

ASJC Scopus subject areas

  • Hepatology

Cite this

Kumamoto, Y., Suematsu, M., Shimazu, M., Kato, Y., Sano, T., Makino, N., ... Kitajima, M. (1999). Kupffer cell-independent acute hepatocellular oxidative stress and decreased bile formation in post-cold-ischemic rat liver. Hepatology, 30(6), 1454-1463.

Kupffer cell-independent acute hepatocellular oxidative stress and decreased bile formation in post-cold-ischemic rat liver. / Kumamoto, Yusuke; Suematsu, Makoto; Shimazu, Motohide; Kato, Yutaro; Sano, Tsuyoshi; Makino, Nobuya; Hirano, Ken Ichiro; Naito, Makoto; Wakabayashi, G. O.; Ishimura, Yuzuru; Kitajima, Masaki.

In: Hepatology, Vol. 30, No. 6, 1999, p. 1454-1463.

Research output: Contribution to journalArticle

Kumamoto, Y, Suematsu, M, Shimazu, M, Kato, Y, Sano, T, Makino, N, Hirano, KI, Naito, M, Wakabayashi, GO, Ishimura, Y & Kitajima, M 1999, 'Kupffer cell-independent acute hepatocellular oxidative stress and decreased bile formation in post-cold-ischemic rat liver', Hepatology, vol. 30, no. 6, pp. 1454-1463.
Kumamoto Y, Suematsu M, Shimazu M, Kato Y, Sano T, Makino N et al. Kupffer cell-independent acute hepatocellular oxidative stress and decreased bile formation in post-cold-ischemic rat liver. Hepatology. 1999;30(6):1454-1463.
Kumamoto, Yusuke ; Suematsu, Makoto ; Shimazu, Motohide ; Kato, Yutaro ; Sano, Tsuyoshi ; Makino, Nobuya ; Hirano, Ken Ichiro ; Naito, Makoto ; Wakabayashi, G. O. ; Ishimura, Yuzuru ; Kitajima, Masaki. / Kupffer cell-independent acute hepatocellular oxidative stress and decreased bile formation in post-cold-ischemic rat liver. In: Hepatology. 1999 ; Vol. 30, No. 6. pp. 1454-1463.
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AU - Sano, Tsuyoshi

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AU - Hirano, Ken Ichiro

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