Kupffer cell-mediated oxidative stress on colon cancer cell line visualized by digital imaging fluorescence microscopy

I. Kurose, H. Saito, M. Suematsu, D. Fukumura, S. Miura, T. Morizane, M. Tsuchiya

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Temporal and spatial changes of lipid peroxides in a cultured colon cancer cell line, Colo-205 cells, were investigated after culturing with Kupffer cells by using 2′,7′-dichlorofluorescein diacetate and a digital imaging processor equipped with an inverted microscope. By this method, we successfully visualized the alteration of lipid peroxides in the individual cancer cell. Without any prior activation, Kupffer cells isolated from an intact rat liver caused rapid increase in the intensity of dichlorofluorescein in tumor cells in a time-dependent manner. The increase of the fluorescent intensity was significantly attenuated by pretreatment with superoxide dismutase. In ex vivo study using isolated perfused rat liver, dichlorofluorescin-preloaded cancer cells, which were transportally injected, were found to adhere to hepatic sinusoids and then to enhance their fluorescence. The present study suggested that the resident Kupffer cell-derived oxidative stress participates in the cytotoxic process against cancer cells by inducing intracellular lipid peroxidation. It may be sustained that Kupffer cells play a role in the host defence mechanisms against the liver metastasis of colon cancer cells.

Original languageEnglish
Pages (from-to)201-209
Number of pages9
JournalCancer Letters
Volume59
Issue number3
DOIs
Publication statusPublished - 1991 Sep

Keywords

  • Kupffer cell
  • colon cancer
  • dichlorofluorescin-diacetate
  • lipid peroxidation
  • oxidative stress

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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