Abstract
Background & Aims: Tissue recruitment of dendritic cells (DCs) is essential for antigen presentation. This study aimed to examine cellular and molecular mechanisms for DC recruitment to the liver. Methods: Purified rat DCs were injected into circulation and their traffics were analyzed in normal and Kupffer cell-depleted rats by intravital confocal microscopy and immunohistology. Affinities of DCs to sinusoidal cells were examined by a cell-binding assay. DC precursor recruitment was induced by particulate injection. Results: Both DC precursors and DCs at the antigen-transporting stage could be recruited to the liver, and their majority initially showed a selective binding to Kupffer cells. In the Kupffer cell-depleted rats, DCs could neither be recruited to the liver nor adhere to sinusoidal walls. Pretreatment with varied monosaccharides showed that sugar residues consisting of N-acetylgalactosamine were necessary for this binding. The binding was calcium-dependent, implying the C-type lectin involvement. Furthermore, DCs could endocytose N-acetylgalactosamine polymers in a receptor-specific manner. Conclusions: The DC-Kupffer cell binding through N-acetylgalactosamine-specific C-type lectin-like receptors is crucial for DC recruitment to the, liver. Rat DCs at least partly possess receptors for endocytosis of galactosylated antigens. These DC receptors as well as Kupffer cell lectins are presumably responsible for this binding.
| Original language | English |
|---|---|
| Pages (from-to) | 1460-1472 |
| Number of pages | 13 |
| Journal | Gastroenterology |
| Volume | 121 |
| Issue number | 6 |
| Publication status | Published - 2001 |
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ASJC Scopus subject areas
- Gastroenterology
Cite this
Kupffer cell-mediated recruitment of rat dendritic cells to the liver : Roles of N-acetylgalactosamine-specific sugar receptors. / Uwatoku, Ryosuke; Suematsu, Makoto; Ezaki, Taichi; Saiki, Takahito; Tsuiji, Makoto; Irimura, Tatsuro; Kawada, Norifumi; Suganuma, Tatsuo; Naito, Makoto; Ando, Masayasu; Matsuno, Kenjiro.
In: Gastroenterology, Vol. 121, No. 6, 2001, p. 1460-1472.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kupffer cell-mediated recruitment of rat dendritic cells to the liver
T2 - Roles of N-acetylgalactosamine-specific sugar receptors
AU - Uwatoku, Ryosuke
AU - Suematsu, Makoto
AU - Ezaki, Taichi
AU - Saiki, Takahito
AU - Tsuiji, Makoto
AU - Irimura, Tatsuro
AU - Kawada, Norifumi
AU - Suganuma, Tatsuo
AU - Naito, Makoto
AU - Ando, Masayasu
AU - Matsuno, Kenjiro
PY - 2001
Y1 - 2001
N2 - Background & Aims: Tissue recruitment of dendritic cells (DCs) is essential for antigen presentation. This study aimed to examine cellular and molecular mechanisms for DC recruitment to the liver. Methods: Purified rat DCs were injected into circulation and their traffics were analyzed in normal and Kupffer cell-depleted rats by intravital confocal microscopy and immunohistology. Affinities of DCs to sinusoidal cells were examined by a cell-binding assay. DC precursor recruitment was induced by particulate injection. Results: Both DC precursors and DCs at the antigen-transporting stage could be recruited to the liver, and their majority initially showed a selective binding to Kupffer cells. In the Kupffer cell-depleted rats, DCs could neither be recruited to the liver nor adhere to sinusoidal walls. Pretreatment with varied monosaccharides showed that sugar residues consisting of N-acetylgalactosamine were necessary for this binding. The binding was calcium-dependent, implying the C-type lectin involvement. Furthermore, DCs could endocytose N-acetylgalactosamine polymers in a receptor-specific manner. Conclusions: The DC-Kupffer cell binding through N-acetylgalactosamine-specific C-type lectin-like receptors is crucial for DC recruitment to the, liver. Rat DCs at least partly possess receptors for endocytosis of galactosylated antigens. These DC receptors as well as Kupffer cell lectins are presumably responsible for this binding.
AB - Background & Aims: Tissue recruitment of dendritic cells (DCs) is essential for antigen presentation. This study aimed to examine cellular and molecular mechanisms for DC recruitment to the liver. Methods: Purified rat DCs were injected into circulation and their traffics were analyzed in normal and Kupffer cell-depleted rats by intravital confocal microscopy and immunohistology. Affinities of DCs to sinusoidal cells were examined by a cell-binding assay. DC precursor recruitment was induced by particulate injection. Results: Both DC precursors and DCs at the antigen-transporting stage could be recruited to the liver, and their majority initially showed a selective binding to Kupffer cells. In the Kupffer cell-depleted rats, DCs could neither be recruited to the liver nor adhere to sinusoidal walls. Pretreatment with varied monosaccharides showed that sugar residues consisting of N-acetylgalactosamine were necessary for this binding. The binding was calcium-dependent, implying the C-type lectin involvement. Furthermore, DCs could endocytose N-acetylgalactosamine polymers in a receptor-specific manner. Conclusions: The DC-Kupffer cell binding through N-acetylgalactosamine-specific C-type lectin-like receptors is crucial for DC recruitment to the, liver. Rat DCs at least partly possess receptors for endocytosis of galactosylated antigens. These DC receptors as well as Kupffer cell lectins are presumably responsible for this binding.
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M3 - Article
C2 - 11729125
AN - SCOPUS:0035207270
VL - 121
SP - 1460
EP - 1472
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 6
ER -