L-glutamate biosensor for estimation of the taste of tomato specimens

Rasa Pauliukaite, Gleb Zhylyak, Daniel Citterio, Ursula E. Spichiger-Keller

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An amperometric biosensor has been developed for measurement of Umami, or the taste based on the amount of L-glutamate, in tomato foods. The biosensor is based on an enzyme-mediator system in which L-glutamate oxidase is used for biochemical oxidation of L-glutamate and a tetrafulvalene- tetracyanoquinodimethane (TTF-TCNQ) paste, prepared from the mixture of TTF-TCNQ salt, graphite powder, and silicone oil, serves as the mediator. The limit of detection, calculated by use of a four-parameter logistic model, was 0.05 mmol L-1, and the limit of quantification was 0.15 mmol L-1. The correlation coefficient (R2) was 0.990 and the relative standard deviation was no more than 1% (n=5). The response time (t95) was 20-50 s, depending on concentration. The repeatability of the sensor was better than 5% (n=10). The sensor developed was stable for more than ten days.

Original languageEnglish
Pages (from-to)220-227
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number3
DOIs
Publication statusPublished - 2006 Oct
Externally publishedYes

Fingerprint

L-glutamate oxidase
Biosensing Techniques
Lycopersicon esculentum
Biosensors
Glutamic Acid
Silicone Oils
Graphite
Sensors
Ointments
Powders
Reaction Time
Limit of Detection
Logistics
Salts
Logistic Models
Food
Oxidation
Enzymes
tetracyanoquinodimethane

Keywords

  • Amperometric biosensors
  • L-Glutamate determination
  • L-Glutamate oxidase
  • TTF-TCNQ
  • Umami

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

L-glutamate biosensor for estimation of the taste of tomato specimens. / Pauliukaite, Rasa; Zhylyak, Gleb; Citterio, Daniel; Spichiger-Keller, Ursula E.

In: Analytical and Bioanalytical Chemistry, Vol. 386, No. 3, 10.2006, p. 220-227.

Research output: Contribution to journalArticle

Pauliukaite, Rasa ; Zhylyak, Gleb ; Citterio, Daniel ; Spichiger-Keller, Ursula E. / L-glutamate biosensor for estimation of the taste of tomato specimens. In: Analytical and Bioanalytical Chemistry. 2006 ; Vol. 386, No. 3. pp. 220-227.
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